安徽中医药大学学报2023，Vol.42Issue(6)：59-65,7.DOI:10.3969/j.issn.2095-7246.2023.06.014
瓜蒌薤白对高脂血症小鼠肝脏三酰甘油合成及SIRT1/AMPK通路的影响
Effect of Fructus Trichosanthis-Macrostem Onion on Hepatic Triglyceride Synthesis and the SIRT1/AMPK Pathway in Hyperlipidemia Mice
摘要
Abstract
Objective To investigate the effect of Fructus Trichosanthis-macrostem onion on hepatic triglyceride(TG)synthesis and the SIRT1/AMPK pathway in hyperlipidemia mice.Methods A total of 40 mice with apolipoprotein E gene knockout were given high-fat feed for 20 weeks and were then randomly divided into model group,atorvastatin group,and low-,middle-,and high-dose Fructus Trichosanthis-macrostem onion groups,and 8 C57BL/6J mice with the same genetic background were en-rolled as blank group.The enzymatic method was used to measure the serum levels of total cholesterol(TC),low-density lipo-protein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C);ELISA and qRT-PCR were used to measure the content and mRNA expression levels of fatty acid synthase(FAS)and acetyl-CoA carboxylase(ACC)in mouse liver;Western blot and qRT-PCR were used to measure the protein and mRNA expression levels of sterol regulatory element-binding protein-1(SREBP-1),carbohydrate response element-binding protein(ChREBP),silent information regulator 1(SIRT1),and AMP-activated protein kinase(AMPK)in mouse liver.Results Fructus Trichosanthis-macrostem onion reduced the serum levels of TG,TC,and LDL-C and increased the serum level of HDL-C.It also reduced the content and mRNA expression lev-els of the hepatic TG synthases FAS and ACC and downregulated the expression levels of the hepatic TG synthesis proteins SREBP-1 and ChREBP.Moreover,it significantly upregulated the expression level of SIRT1 and downregulated the expression level of AMPK.Conclusion Fructus Trichosanthis-macrostem onion can regulate blood lipid levels in hyperlipidemia mice pos-sibly by activating the SIRT1/AMPK pathway and inhibiting the expression of proteins and synthases associated with hepatic TG synthesis and the endogenous synthesis of TG.关键词
瓜蒌/薤白/三酰甘油/脂肪合成/信号通路/SIRT1/AMPKKey words
Fructus Trichosanthis/Macrostem onion/Triglyceride/Fat synthesis/Signaling pathway/Silent information reg-ulator 1/AMP-activated protein kinase分类
医药卫生引用本文复制引用
刘洁,吴鸿飞,王雨婷,李正龙,张文龙,许添阳,谢宜瑜,王清萍,陈颖,吴冰雨..瓜蒌薤白对高脂血症小鼠肝脏三酰甘油合成及SIRT1/AMPK通路的影响[J].安徽中医药大学学报,2023,42(6):59-65,7.基金项目
安徽省自然科学基金项目(2208085MH275) （2208085MH275）
安徽高校自然科学研究项目(KJ2021A0592) （KJ2021A0592）
安徽省大学生创新创业基金(S202110369032,S202110369046) （S202110369032,S202110369046）
