安徽中医药大学学报2023，Vol.42Issue(6)：65-72,8.DOI:10.3969/j.issn.2095-7246.2023.06.015
灵芝酸治疗阿尔茨海默病作用机制的网络药理学研究
Mechanism of Action of Ganoderic Acid in Treatment of Alzheimer's Disease:A Study Based on Network Pharmacology
摘要
Abstract
Objective To investigate the potential mechanism of action of ganoderic acid in the treatment of Alzheimer's disease(AD)by identifying related targets based on network pharmacology.Methods The Swiss Target Prediction platform was used to predict the targets of ganoderic acid,and DisGeNET,DrugBank,and GeneCards databases were used to search for the tar-gets related to AD;these two groups of targets were intersected to obtain the potential targets of ganoderic acid acting on AD.The STRING platform was used to construct a protein-protein interaction(PPI)network,and network topology was used to i-dentify the core targets;Bioconductor bioinformatics software was used to perform GO and KEGG pathway enrichment analy-ses of the intersecting targets to screen for the signaling pathways with a relatively high degree of association with ganoderic acid and AD.A cell experiment was conducted to validate the targets with the highest degree of enrichment and further clarify the core targets of ganoderic acid acting on AD.Results A total of 1 211 targets were obtained for ganoderic acid and AD in databases,among which there were 56 common targets.The PPI network topological analysis showed that the key targets of ganoderic acid in the treatment of AD included AKT1,ALB,MAPK3,CASP3,and TNF.The KEGG pathway enrichment anal-ysis showed that the pathways of AD,neural degeneration,human macrophage infection,and Kaposi's sarcoma-related herpes virus were mainly involved in the treatment of AD by ganoderic acid.The cell experiment showed that at the optimal concentra-tionof 50μmol/L,ganoderic acid A could protect PC12 cells against the injury caused by Aβ25-35 by inhibiting the expressionof p-PI3Kand p-AKT.Conclusion Ganoderic acid exerts a targeted therapeutic effect on AD possibly by key proteins and impor-tant pathways.关键词
灵芝酸/阿尔茨海默病/网络药理学/PI3K/AKT/信号通路Key words
Ganoderic acid/Alzheimer's disease/Network pharmacology/PI3K/AKT signaling pathway分类
医药卫生引用本文复制引用
覃云鹏,邵楠,叶树,宋航,蔡标..灵芝酸治疗阿尔茨海默病作用机制的网络药理学研究[J].安徽中医药大学学报,2023,42(6):65-72,8.基金项目
高校学科(专业)拔尖人才学术资助项目(gxbj2022024) （专业）
安徽中医药大学2022年度第一批人才支持计划项目(2022rcyb028) （2022rcyb028）
