安徽中医药大学学报2023，Vol.42Issue(6)：73-79,7.DOI:10.3969/j.issn.2095-7246.2023.06.016
心通口服液治疗急性心肌梗死的代谢组学研究
Mechanism of Action of Xintong Oral Liquid in Treatment of Acute Myocardial Infarction:A Metabolomics Study
摘要
Abstract
Objective To investigate the mechanism of action of Xintong Oral Liquid in the treatment of acute myocardial in-farction(AMI)by using non-targeted metabolomics techniques to identify the differentially expressed metabolites and metabolic pathways in AMI model rats before and after treatment.Methods Ligation of the anterior descending branch of left coronary artery was performed to establish a rat model of AMI,and then the rats were divided into groups and administered to evaluate the therapeutic effect of Xintong Oral Liquid.Ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry was used to perform a metabolomics analysis of serum,and related software and databases were used to identify differentially expressed metabolites and metabolic pathways.Results The observation of pathological sec-tion showed that compared with the model group with disordered arrangement of cardiomyocytes,Xintong Oral Liquid signifi-cantly reversed cardiomyocyte injury caused by AMI.The metabolomics analysis showed that Xintong Oral Liquid induced changes in 16 metabolites including choline,betaine,stearic acid,and palmitic acid,and the enrichment analysis of metabolic pathways showed that the therapeutic effect of Xintong Oral Liquid might be associated with the metabolic pathways such as bi-osynthesis of unsaturated fatty acids,glycine/serine/threonine metabolism,and arachidonic acid metabolism.Conclusion Xin-tong Oral Liquid can significantly improve heart tissue and pathological conditions in AMI rats and achieve the goal of balancing lipid metabolism and amino acid metabolism by regulating related metabolic pathways and metabolites,thereby exerting a thera-peutic effect on AMI.关键词
急性心肌梗死/心通口服液/代谢组学/脂质代谢/氨基酸代谢Key words
Acute myocardial infarction/Xintong Oral Liquid/Metabolomics/Lipid metabolism/Amino acid metabolism分类
医药卫生引用本文复制引用
陶舒悦,梁万徽,汪杰,张云静,方玲,彭灿..心通口服液治疗急性心肌梗死的代谢组学研究[J].安徽中医药大学学报,2023,42(6):73-79,7.基金项目
亳州市科技重大专项揭榜挂帅项目(BZKXJSJ-134) （BZKXJSJ-134）
安徽省科技重大专项(18030801117) （18030801117）
