安徽中医药大学学报2023，Vol.42Issue(6)：79-85,7.DOI:10.3969/j.issn.2095-7246.2023.06.017
桃红四物汤多糖的结构分析及对缺血性脑卒中大鼠的抗炎作用
A Structural Analysis of Taohong Siwu Decoction Polysaccharide and Its Anti-inflammatory Effect in Rats with Ischemic Stroke
摘要
Abstract
Objective To investigate the structural features of Taohong Siwu Decoction polysaccharide(THSWDP)and its anti-in-flammatory effect in rats with ischemic stroke,and to provide a basis for subsequent pharmacodynamic studies.Methods THSWDP was extracted by the water-extraction and alcohol-precipitation method,the phenol-sulfuric acid method was used to measure the content of total sugar in THSWDP,and gel permeation chromatography was used to measure the molecular weight of TH-SWDP.Monosaccharide composition and infrared absorption features were also analyzed.The suture method was used to es-tablish a model of middle cerebral artery occlusion,and neurological score was determined after 24 hours.The rats were ran-domly divided into sham-operation group,model group,Taohong Siwu Decoction group,nimodipine group,and low-,middle-,and high-dose THSWDP groups.After 7 days of administration,Berderson score was used to evaluate neurological function;TTC staining was used to measure cerebral infarction rate;ELISA was used to measure the serum levels of interleukin 1β(IL-1β),interleukin 6(IL-6),and tumor necrosis factor-α(TNF-α);HE staining was used to observe the pathological injury of brain tissue;immunohistochemistry and Western blotting were used to measure the expression of inflammatory factors in brain tissue.Results The content of total sugar was 75.25%in THSWDP,and THSWDP had a molecular weight of 2.47×104 Da and was a heteropolysaccharide composed of mannose,galacturonic acid,rhamnose,glucose,galactose,and fucose.Infrared results showed the characteristic absorption of polysaccharides in the structure of THSWDP.The results of animal experiment showed that after THSWDP intervention,there was an improvement in brain tissue injury,varying degrees of reductions in neurological score and cerebral infarction rate,and significant reductions in the levels of IL-6,IL-1β,and TNF-αin serum and brain tissue.Conclusion THSWDP exerts a cerebral protection effect on a rat model of ischemic stroke by inhibiting inflamma-tory response.关键词
桃红四物汤/多糖结构/单糖组成/缺血性脑卒中Key words
Taohong Siwu Decoction/Polysaccharide structure/Monosaccharide composition/Ischemic stroke分类
医药卫生引用本文复制引用
张亚南,赵梦蝶,唐林峰,侯欣宇,张亚中,韩岚,彭代银..桃红四物汤多糖的结构分析及对缺血性脑卒中大鼠的抗炎作用[J].安徽中医药大学学报,2023,42(6):79-85,7.基金项目
国家自然科学基金项目(8207142977,81903953) （8207142977,81903953）
安徽省高校自然科学研究项目(KJ2020A0420) （KJ2020A0420）
