安徽中医药大学学报2023，Vol.42Issue(6)：86-92,7.DOI:10.3969/j.issn.2095-7246.2023.06.018
茯苓皮总三萜大孔树脂纯化工艺及体外抗炎活性研究
Macroporous Resin Purification Process and in Vitro Anti-inflammatory Activity of Total Triter-penoids from Cortex Poriae Cocos
摘要
Abstract
Objective To establish a simple and convenient method for the purification of total triterpenoids from Cortex Poriae Cocos with the purpose of improving the comprehensive utilization value of Cortex Poriae Cocos,and to investigate its anti-inflammatory effect byin vitro experiments.Methods The method of HPD-100 macroporous resin absorption was used for the purification of total triterpenoids from Cortex Poriae Cocos.Based on the single-factor experiment and the content of total triter-penoids as the index,the orthogonal test was used to optimize the purification process of total triterpenoids from Cortex Poriae Cocos.RAW 264.7 cells were used to establish a model of lipopolysaccharide-induced inflammation,and the effect of total trit-erpenoids from Cortex Poriae Cocos on cell viability was analyzed.After administration,the RAW 264.7 cells were measured in terms of the release of nitric oxide(NO)and the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and inter-leukin-6(IL-6).Results After purification,the content of total triterpenoids in sample increased to 70.36%,which was signif-icantly higher than that of the crude extract of total triterpenoids(29.76%).The purification process was 20 g of HPD-100 res-in on the upper column,a loading concentration of 15 mg/mL,5 bed volumes(BVs)of 35%ethanol-eluted impurity volume,and 4 BVs of 95%ethanol-eluted triterpenoid volume,and the experiment showed that this purification process had good repeat-ability.Thein vitro experiment showed that total triterpenoids from Cortex Poriae Cocos had no significant effect on cell viabili-ty within the range of 50-250μg/mL,and compared with the model group,there were significant reductions in the release of NO and the levels of TNF-α,IL-1β,and IL-6 in RAW 264.7 cells after administration(P＜0.05).Conclusion The purification process of HPD-100 macroporous resin adsorption can significantly increase the content of total triterpenoids in the sample,and the total triterpenoids from Cortex Poriae Cocos can alleviate inflammation.关键词
茯苓皮/三萜/正交试验/HPD-100大孔树脂/纯化/抗炎Key words
Cortex Poriae Cocos/Triterpenoids/Orthogonal test/HPD-100 macroporous resin/Purification/Anti-inflammatory effect分类
医药卫生引用本文复制引用
吕婷婷,王妍妍,张越,孟祥宇,丁卫健,彭代银,王雷,姚亮,陈卫东..茯苓皮总三萜大孔树脂纯化工艺及体外抗炎活性研究[J].安徽中医药大学学报,2023,42(6):86-92,7.基金项目
国家自然科学基金青年基金项目(82204748) （82204748）
中华中医药学会青年人才托举工程项目(CACM-2022-QNRC2-B03) （CACM-2022-QNRC2-B03）
国家中医药管理局高水平中医药重点学科建设项目(国中医药人教函[2023]85号) （国中医药人教函[2023]85号）
