滨州医学院学报2023，Vol.46Issue(6)：401-405,5.DOI:10.19739/j.cnki.issn1001-9510.2023.06.001
RUNX1在AngⅡ诱导心肌肥厚中的作用及机制
Role and mechanism of RUNX1 in Ang Ⅱ-induced cardiac hypertrophy
摘要
Abstract
Objective To investigate the effect of Runt-related transcription factor 1(RUNX1)on cardiac hypertrophy in-duced by angiotensin Ⅱ(AngⅡ)and its mechanism.Methods H9C2 cardiomyocytes were divided into the control group,the L-Ang Ⅱ group,the M-Ang Ⅱ group and the H-Ang Ⅱ group.Ang Ⅱ-induced cardiac hypertrophy model was established and the expression of RUNX1 was detected under different concentrations of Ang Ⅱ.H9C2 cardiomyocytes were divided into the NS group,the Ang Ⅱ+NS group,the shRUNX1 group and the AngⅡ+shRUNX1 group to detect the effect of RUNX1 on cardio-myocyte hypertrophy.Western blot was used to detect the expression of RUNX1 and SERCA2a.qRT-PCR was used to detect the related indexes of cardiac hypertrophy,and immunofluorescence was used to detect the myocardial cell area.Results Compared with that in the control group,the expression of RUNX1 protein in H9C2 cells treated with AngⅡfor 48 h was up-regulated in a dose-dependent manner.Compared with that in the AngⅡ+NS group,the expression of BNP mRNA and RUNX1 protein in the Ang Ⅱ+shRUNX1 group decreased significantly,and the area of cardiomyocytes decreased significantly.The expression of SERCA2a protein was up-regulated.Conclusion RUNX1 is involved in the pathogenesis of Ang Ⅱ-induced cardiac hypertro-phy,and its mechanism may be related to the inhibition of SERCA2a.关键词
心肌肥厚/血管紧张素Ⅱ/Runt相关转录因子1/SERCA2aKey words
cardiac hypertrophy/angiotensin Ⅱ/Runt-related transcription factor 1/SERCA2a分类
医药卫生引用本文复制引用
丁立群,王玉玖,杨丽娟,沈嘉祺,王傲,邹明锐,刘宝辉..RUNX1在AngⅡ诱导心肌肥厚中的作用及机制[J].滨州医学院学报,2023,46(6):401-405,5.基金项目
山东省自然科学基金(ZR2020QH017) （ZR2020QH017）
山东省医药卫生科技发展计划(2019WS327) （2019WS327）
