滨州医学院学报2023，Vol.46Issue(6)：411-415,5.DOI:10.19739/j.cnki.issn1001-9510.2023.06.003
增味小承气汤对梗阻性黄疸大鼠肠道黏膜紧密连接蛋白-1、分泌型免疫球蛋白A及黏蛋白-2表达的影响
Effects of modified Xiaochengqi Decoction on expression of zonulaoccludens-1,secreted immunoglobulin A and mucoprotein-2 in intestinal mucosa of rats with obstructive jaundice
摘要
Abstract
Objective To observe the effects of modified Xiaochengqi Decoction on the expression of the zonulaoccludens-1(ZO-1),secreted immunoglobulin A(s IgA)and mucoprotein-2(MUC-2)in the intestinal mucosa of rats with obstructive jaun-dice,and analyze the effects of modified Xiaochengqi Decoction on intestinal mucosal barrier function.Methods The SD male rats were randomly divided into the control group,the sham group,the surgical model of obstructive jaundice+modified Xi-aochengqi Decoction(OJ+M)group and the surgical model of obstructive jaundice(OJ)group,with 10 rats in each group.Blood was collected via the caudal vein on the 3rd,5th and 7th day after modeling and experimental intervention.All rats were killed on the 8th day after modeling with corresponding specimens collected.The collected intestinal specimens were submitted for HE staining and their small intestinal villous height and crypt depth were measured;the expression sites and levels of ZO-1,s IgA and MUC-2 were detected by immunohistochemistry to reveal the effect of modified Xiaochengqi Decoction on intestinal mu-cosal mechanical barrier,immune barrier,and biological barrier functions.Results Compared with those in the OJ group,the small intestine villus height and the small intestine crypt depth were increased in the OJ+M group.The expression levels of ZO-1,s IgA,and MUC-2 were higher in the OJ+M group.Conclusion Modified Xiaochengqi Decoction increased the expression of ZO-1,s IgA and MUC-2 in intestinal mucosa of rats with obstructive jaundice,and alleviated the damage of intestinal barrier function.关键词
增味小承气汤/梗阻性黄疸/肠黏膜屏障Key words
modified Xiaochengqi Decoction/obstructive jaundice/intestinal mucosal barrier分类
医药卫生引用本文复制引用
魏强,赵雪谦,张帆,孙宝房,冀海斌,陈强谱..增味小承气汤对梗阻性黄疸大鼠肠道黏膜紧密连接蛋白-1、分泌型免疫球蛋白A及黏蛋白-2表达的影响[J].滨州医学院学报,2023,46(6):411-415,5.基金项目
滨州医学院科技计划(BJ2018KJ11) （BJ2018KJ11）
