滨州医学院学报2023，Vol.46Issue(6)：416-419,4.DOI:10.19739/j.cnki.issn1001-9510.2023.06.004
Wnt2基因启动子DNA甲基化在子痫前期发病机制中的作用
Role of DNA methylation in the promoter region of Wnt2 gene in the pathogenesis of preeclampsia
摘要
Abstract
Objective To detect DNA methylation degree in the promoter region of Wnt2 gene in the placenta,and explore the relationship between Wnt2 gene and preeclampsia.Methods Data were collected from the Department of Gynaecology and Obstetrics.Thirty patients with early-onset severe preeclampsia were treated as the early-onset severe preeclampsia group(ZPE),30 patients with late-onset severe preeclampsia were treated as the late-onset severe preeclampsia group(WPE),and 30 normal late pregnancy women during the same period were treated as the control group(NC).All pregnant women in the study were delivered by cesarean section.Methylation-specific polymerase chain reaction was used to detect the DNA methylation in the Wnt2 gene promoter region in placenta.Results Compared with that in the NC group,the DNA methylation level of Wnt2 gene promoter region in the ZPE group(P＜0.05),and there was no statistically significant difference in DNA methylation in the Wnt2 promoter region between the early-onset severe preeclampsia group(ZPE)and the late-onset severe preeclampsia group(WPE).Conclusion Incomplete DNA methylation in the Wnt2 promoter region was found in the placentas of normal women in the third trimester,early-onset severe preeclampsia and late-onset severe preeclampsia.Wnt2 promoter DNA methylation was sig-nificantly increased in the placentas of patients with early-onset severe preeclampsia and late-onset severe preeclampsia compared with that of normal late pregnant women.关键词
Wnt2基因/表观遗传学/甲基化/子痫前期Key words
Wnt2 gene/epigenetics/methylation/preeclampsia分类
医药卫生引用本文复制引用
刘玉芳,崔春燕,张振涛,王云芳..Wnt2基因启动子DNA甲基化在子痫前期发病机制中的作用[J].滨州医学院学报,2023,46(6):416-419,4.基金项目
山东省医药卫生科技发展计划(202005020624) （202005020624）
山东省自然科学基金(ZR2021MH247) （ZR2021MH247）
