滨州医学院学报2023，Vol.46Issue(6)：425-429,5.DOI:10.19739/j.cnki.issn1001-9510.2023.06.006
表没食子儿茶素没食子酸酯通过抑制NLRP3炎症小体改善溃疡性结肠炎小鼠肠道屏障的研究
EGCG improves intestinal barrier in mice with ulcerative colitis by inhibiting NLRP3 inflammasome
摘要
Abstract
Objective To investigate the effect of epigallocatechin-3-gallate(EGCG)on the intestinal barrier in ulcerative colitis mice and explore its mechanism.Methods Male C57BL/6J mice were randomly divided into the normal(CON)group,the model(DSS)group,the model+low-dose EGCG(LEG)group,and the model+high-dose EGCG(HEG)group,with 8 mice in each group.A mouse model of ulcerative colitis(UC)was established by administering drinking water containing 3%dextran sulfate sodium.The LEG and HEG groups were gavaged with 50 and 100 mg/kg EGCG,while the CON and DSS groups were gavaged with normal saline.After 7 days,the levels of disease activity index(DAI),colon length,and IL-1β,IL-6 and IL-10 in serum were detected.The pathological changes of colon tissue were observed by H&E staining.The mRNA expression of zonulaoccludens-1(ZO-1)and occludin in colon tissue was detected using RT-qPCR.The protein expression levels of nucleo-tide-binding oligomerization domain-like receptor protein 3(NLRP3)and caspase-1 were detected using Western blot.Results Compared with the CON group,the DSS group had a shortened colon length.DAI score,serum pro-inflammatory factor levels,and NLRP3 and caspase-1 protein expression were significantly increased.The concentration of serum IL-10 and the levels of ZO-1 and occludin mRNA expression were decreased.Compared with the DSS group,EGCG treatment showed improvements in the above indicators in UC mice.Conclusion EGCG can improve colonic barrier damage and inflammatory response in UC mice,and the mechanisms might be associated with the inhibition of the NLRP3 inflammasome activation.关键词
表没食子儿茶素没食子酸酯/溃疡性结肠炎/结肠/肠道屏障/NLRP3炎症小体Key words
epigallocatechin-3-gallate/ulcerative colitis/colon/intestinal barrier/NLRP3 inflammasome分类
医药卫生引用本文复制引用
张敏,张晟,周华..表没食子儿茶素没食子酸酯通过抑制NLRP3炎症小体改善溃疡性结肠炎小鼠肠道屏障的研究[J].滨州医学院学报,2023,46(6):425-429,5.基金项目
安徽省高等学校自然科学研究项目(ZR2022B007) （ZR2022B007）
