川北医学院学报2023，Vol.38Issue(12)：1587-1591,5.DOI:10.3969/j.issn.1005-3697.2023.12.001
7种不同类型肿瘤组织内胱抑素B表达的比较研究
Comparative study of cystatin B expression in tissues of 7 different types of tumors
摘要
Abstract
Objective:To compare the expression of cyatatin B(CSTS)in 7 different types of cancer tissues with corresponding adjacent tissues.Methods:Cancer tissues and paired adjacent non-cancerous tissues were collected from patients with esophageal squa-mous cell carcinoma,laryngeal squamous cell carcinoma,gastric adenocarcinoma,lung squamous cell carcinoma,hepatocellular carcino-ma,colon adenocarcinoma,and bladder transitional cell carcinoma.The esophageal cancer cell line Eca109,gastric cancer cell line SGC7901,liver cancer cell line Hep G2,and lung cancer cell line YTLMC-90 were cultured in vitro.Western blot analysis was used to assess the expression levels of CSTB in esophageal cancer tissues and corresponding adjacent mucosal tissues.Immunohistochemistry was performed to detect the expression of CSTB in various tissue specimens and cancer cell lines.Results:CSTB was expressed in the cultured cancer cell lines,including Eca109,SGC7901,Hep G2,and YTLMC-90.Among the adjacent non-cancerous tissues,CSTB was expressed in adjacent laryngeal mucosa,esophageal mucosa,gastric mucosa,and liver tissues.Other adjacent non-cancerous tissues showed low expression or no expression of CSTB.However,in the corresponding cancer tissues,CSTB expression was decreased in laryn-geal squamous cell carcinoma,esophageal squamous cell carcinoma,and gastric adenocarcinoma(P<0.05),while it remained highly expressed in liver cancer tissues(P<0.05).CSTB expression was increased in lung squamous cell carcinoma,colon adenocarcinoma,and bladder transitional cell carcinoma(P<0.05).Conclusion:These findings confirmed the dysregulation of CSTB expression in dif-ferent types of solid cancers,providing a molecular basis for further elucidation of the mechanisms of different types of tumor develop-ment.关键词
胱抑素B/癌症/表达Key words
Cystatin B/Cancer/Expression分类
医药卫生引用本文复制引用
敬昊东,冯莉,王朝莉,李丽,谢勇恩,胡为民,杨健,敬保迁..7种不同类型肿瘤组织内胱抑素B表达的比较研究[J].川北医学院学报,2023,38(12):1587-1591,5.基金项目
国家自然科学基金(30872213) （30872213）
川北医学院青年基金(CBY22-QNA14) （CBY22-QNA14）
