川北医学院学报2023，Vol.38Issue(12)：1592-1597,6.DOI:10.3969/j.issn.1005-3697.2023.12.002
芒柄花素调节Nrf2/HO-1信号通路对急性肺栓塞大鼠肺组织损伤的影响
Influence of formononetin on lung tissue damage in rats with acute pulmo-nary embolism by regulating Nrf2/HO-1 signaling pathway
摘要
Abstract
Objective:To investigate the influence of formononetin(FN)on lung tissue damage in rats with acute pulmonary embolism(APE)by regulating nuclear factor E2-related factor 2/heme oxygenase-1(Nrf2/HO-1)signaling pathway.Methods:SD rats were randomly divided into control group,APE model group(APE group),low molecular weight heparin calcium group(LMWH group),low-dose FN group(L-FN group),high-dose FN group(H-FN group),Nrf2 inhibitor Brusatol group(BR group),and high-dose FN +Brusatol group(H-FN +BR group)using a random number table method,12 cases in each group.APE model was construc-ted by autothrombus transfusion.After 7 days of intervention with corresponding drugs or physiological saline,arterial partial pressure of oxygen(PaO2),oxygenation index(OI)and the serum levels of B-type natriuretic peptide(BNP),troponin Ⅰ(TnⅠ),ischemia-modified albumin(IMA)were measured,lung tissue pathology was detected by HE staining,the levels of interleukin-6(IL-6)and tumor necrosis factor(TNF)-α in serum were determined by ELISA,kits were used to measure the levels of superoxide dismutase(SOD),and malondialdehyde(MDA)in lung tissue,Western blot was applied to detect the protein levels of Nrf2,HO-1,NF-κB,and p-NF-κB in lung tissue.Results:Compared with the control group,the lung tissue structure of the APE group was disordered,the alveoli were ruptured and fused,with exudate,the interstitial area was edema and a large number of inflammatory cells were infiltrated,the lev-els of PaO2,OI and SOD,Nrf2,HO-1 in lung tissue were decreased,the serum levels of BNP,TnⅠ,IMA,IL-6,TNF-α,and MDA,p-NF-κB/NF-κB in lung tissue were increased(P<0.05).Compared with the APE group,the lung tissue lesions of the LMWH group,L-FN group and H-FN group were alleviated,the levels of PaO2,OI and SOD,Nrf2,HO-1 in lung tissue were increased,and the L-FN group was the lowest among the groups(P<0.05).The serum levels of BNP,TnⅠ,IMA,IL-6,TNF-α,and MDA,p-NF-κB/NF-κB in lung tissue were decreased,and the L-FN group was the highest among the groups(P<0.05),the above indexes in BR group showed reverse changes(P<0.05).Compared with the H-FN group,the pathological changes of the lung tissue of the H-FN +BR group were aggravated,the inflammatory cells increased,the levels of PaO2,OI and SOD,Nrf2,HO-1 in lung tissue were decreased,the serum levels of BNP,TnⅠ,IMA,IL-6,TNF-α,and MDA,p-NF-κB/NF-κB in lung tissue were increased(P<0.05).Conclusion:FN may alleviate lung tissue damage in APE rats by activating the Nrf2/HO-1 pathway.关键词
芒柄花素/核因子E2相关因子2/血红素加氧酶-1/急性肺栓塞/肺损伤Key words
Formononetin/Nuclear factor E2-related factor 2/heme oxygenase-1/Acute pulmonary embolism/Lung damage分类
医药卫生引用本文复制引用
赵博韬,毛宏宇,姚春霞,吴清华..芒柄花素调节Nrf2/HO-1信号通路对急性肺栓塞大鼠肺组织损伤的影响[J].川北医学院学报,2023,38(12):1592-1597,6.基金项目
河北省自然科学基金资助项目(H2020402004) （H2020402004）
河北省沧州市科技局科技研究与发展指导计划(172302180) （172302180）
