川北医学院学报2023，Vol.38Issue(12)：1598-1602,5.DOI:10.3969/j.issn.1005-3697.2023.12.003
基于VEGF/VEGFR信号通路探讨贝伐珠单抗对肠癌SW480细胞生物学行为的影响及其机制
Exploration on effect and mechanism of bevacizumab on the biological be-haviors of intestinal cancer SW480 cells based on the VEGF/VEGFR sig-nal pathway
摘要
Abstract
Objective:To explore the effect and mechanism of bevacizumab on the biological behaviors of intestinal cancer cells based on vascular endothelial growth factor(VEGF)/vascular endothelial growth factor receptor(VEGFR)signaling pathway.Meth-ods:The human intestinal cancer cell line SW480 cells were selected as the subjects of this study.The SW480 cells were treated with bevacizumab at different concentrations(0,25,50,100,200 μg/mL),and Cell Counting Kit-8(CCK8)was used to detect the cell pro-liferation level,and Transwell migration and invasion test was applied to detect the cell migration and invasion abilities.Fluorescein iso-thiocyanate(Annexin V-FITC)double staining method was adopted to measure the cell apoptosis,and Western Blot was for the detec-tion of protein expression levels of intracellular VEGF,VEGFR1 and VEGFR2.The effect of different concentrations of bevacizumab on proliferation,migration,invasion,apoptosis and VEGF/VEGFR signaling pathway in colorectal cancer cells was compared.Results:Bev-acizumab inhibited the in vitro proliferation,migration and invasion of intestinal cancer cells in a concentration-dependent manner(P<0.05).Bevacizumab induced the in vitro apoptosis of intestinal cancer cells in a concentration-dependent manner(P<0.05).As the concentration of bevacizumab increased,the expression of VEGF was down-regulated while the expressions of VEGFR1 and VEGFR2 were up-regulated(P<0.05).Conclusion:Bevacizumab can inhibit the proliferation,migration and invasion and induce the cell apop-tosis of intestinal cancer cells in vitro by regulating the VEGF/VEGFR signaling pathway.关键词
血管内皮生长因子/血管内皮生长因子受体/贝伐珠单抗/肠癌/细胞生物学行为Key words
Vascular endothelial growth factor/Vascular endothelial growth factor receptor/Bevacizumab/Intestinal cancer/Cell biological behaviors分类
医药卫生引用本文复制引用
樊叶,李超,汪志兵..基于VEGF/VEGFR信号通路探讨贝伐珠单抗对肠癌SW480细胞生物学行为的影响及其机制[J].川北医学院学报,2023,38(12):1598-1602,5.基金项目
江苏省南京市卫生科技发展专项资金项目(ZKX21042) （ZKX21042）
