重庆医学2023，Vol.52Issue(23)：3538-3545,8.DOI:10.3969/j.issn.1671-8348.2023.23.004
内皮祖细胞损伤来源微粒对内皮祖细胞的影响机制研究
Study on the mechanism of the effect of endothelial progenitor cell injury-derived microparticles on endothelial progenitor cells
摘要
Abstract
Objective To investigate the effects of endothelial progenitor cells(EPC)-derived microp-articles(MPs)with different injury treatments on EPC.Methods EPC cells were cultured and EPCs were i-dentified by flow cytometry.EPCs were treated with high glucose(HG)and tumor necrosis factor(TNF)-a recombinant protein,and MPs were extracted.The structural changes of microtubules,Golgi bodies and other organelles in EPC were observed by transmission electron microscopy.MTT assay was used to detect cell pro-liferation.Cell scratch assay was used to evaluate cell migration ability.Cell lumen formation assay was used to detect lumen formation.The expression levels of endothelial nitric oxide synthase(eNOS),silent informa-tion regulator 1(SIRT1),rat sarcoma(RAS)and extracellular regulated protein kinase(ERK)in EPC after different treatments were detected by quantitative real-time fluorescence PCR(qPCR)and Western blot.Re-sults Compared with the control-EPC-MPs group,the microtubule structure of the HG-EPC-MPs group and the TNF-α-EPC-MPs group was complete,the length was shortened,and the Golgi structure was relatively complete.Compared with the control-EPC-MPs group,the proliferation rate of EPC in the HG-EPC-MPs group and the TNF-α-EPC-MPs group was down-regulated,the cell migration ability was decreased,and the tube formation was decreased(P＜0.05).Compared with the control-EPC-MPs group,the mRNA and protein expressions of eNOS,SIRT1,RAS and ERK in the HG-EPC-MPs group and the TNF-α-EPC-MPs group were down-regula-ted(P＜0.05).Conclusion HG and TNF-α mediated MPs derived from EPC injury may change the structure of organelles in EPC by regulating the expression of SIRT1/ERK1 pathway proteins,thus affecting the biolog-ical function of EPC.关键词
内皮祖细胞/微粒/SIRT1/ERK1/细胞损伤/机制研究Key words
endothelial progenitor cells/microparticles/SIRT1/ERK1/cell damage/mechanism re-search分类
医药卫生引用本文复制引用
马艺萍,阿不都热合曼·米吉提,袁玉娟,吐尔孙阿依·依斯米提拉,阿卜拉江·艾合麦提,穆叶赛·尼加提..内皮祖细胞损伤来源微粒对内皮祖细胞的影响机制研究[J].重庆医学,2023,52(23):3538-3545,8.基金项目
国家自然科学基金地区基金项目(82060076) （82060076）
新疆维吾尔自治区研究生创新项目(XJ2023G202). （XJ2023G202）
