大连工业大学学报2023，Vol.42Issue(6)：409-413,5.DOI:10.19670/j.cnki.dlgydxxb.2023.0604
群体感应淬灭酶AiiO的克隆表达及其发酵工艺优化
Cloning and expression of quorum sensing quenching enzyme AiiO and optimization of fermentation process
摘要
Abstract
The quorum sensing quenching enzyme AiiO has the activity of degrading signal molecule N-acyl homoserine lactone(AHL),inhibiting the pathogenicity of pathogens.To increase the expression level of AiiO,a recombinant strain Escherichia coli BL21(DE3)-pET-28a(+)-aiiO was constructed.The induction temperature,nitrogen source content,induction timing,and lactose addition during its fermentation production of AiiO were optimized.The results showed that under the optimized conditions of inducing temperature of 25 ℃,peptone of 20 g/L and yeast powder of 10 g/L,adding lactose at the beginning of fermentation,and lactose addition of 5 g/L,the expression level of intracellular soluble AiiO reached 374.26 mg/L.关键词
群体感应淬灭酶/克隆表达/发酵工艺/优化Key words
quorum quenching enzyme AiiO/cloning expression/fermentation process/optimization分类
生物科学引用本文复制引用
田唱,郭婉萍,陈阳,赵晶,陈明..群体感应淬灭酶AiiO的克隆表达及其发酵工艺优化[J].大连工业大学学报,2023,42(6):409-413,5.基金项目
辽宁省高等学校创新人才支持计划(LT2019001). （LT2019001）
