大连工业大学学报2023，Vol.42Issue(6)：414-419,6.DOI:10.19670/j.cnki.dlgydxxb.2023.0605
虎杖中白藜芦醇提取工艺的优化及其抗氧化性
Optimization of extraction process and antioxidant activity of resveratrol from Polygonum cuspidatum
摘要
Abstract
The effects of ultrasound power,extraction time,ethanol volume fraction,and solid-liquid ratio on the extraction process of resveratrol from Polygonum cuspidatum were investigated using single factor and response surface experiments.A combination of macroporous adsorption resin and reverse phase column chromatography was used to separate and identify resveratrol by HPLC.The intracellular and extracellular antioxidant studies were conducted,as well as protective effects on fibroblasts under UV irradiation.The optimal extraction conditions for resveratrol were ultrasound power of 496 W,extraction time of 33 min,ethanol volume fraction of 64％,and solid-liquid ratio of 1∶17.Under the optimal conditions,the extraction rate was 4.80 mg/g and the purity of the obtained resveratrol reached 99.90％.When the contents of resveratrol were 2.5 and 0.5 mg/mL,DPPH and hydroxyl radical scavenging rates reached the highest of 94.92％and 94.65％,and the IC50 were 0.926 and 0.165 mg/mL,respectively.Resveratrol can significantly reduce SOD,CAT,GSH and MDA in skin fibroblasts exposed to ultraviolet radiation.关键词
虎杖/白藜芦醇/抗氧化性/细胞保护Key words
Poly gonum cuspidatum/resveratrol/antioxidant ability/cytoprotection分类
生物科学引用本文复制引用
苏东海,张虎成,彭坚,杨国伟,辛秀兰..虎杖中白藜芦醇提取工艺的优化及其抗氧化性[J].大连工业大学学报,2023,42(6):414-419,6.基金项目
第二批国家职业教育教师创新团队课题研究项目(ZH2021050101). （ZH2021050101）
