分析测试学报2023，Vol.42Issue(12)：1547-1557,11.DOI:10.19969/j.fxcsxb.23072502
基于UPLC-Q-Orbitrap-HRMS技术鉴定护心胆中异喹啉类生物碱成分
Identification of Isoquinoline Alkaloids Components in Corydalis Suaveolens Hance Based on UPLC-Q-Orbitrap-HRMS Technique
摘要
Abstract
This study applied ultra performance liquid chromatography tandem quadrupole electro-static field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap-HRMS)to analyze and identify isoquinoline alkaloids in the roots of Corydalis suaveolens Hance.The methanol extract of Co-rydalis suaveolens processed with a Poroshell RRHD C18 column(150 mm×2.1 mm,1.8 μm)with acetonitrile and 0.1%formic acid water solution as the mobile phase for gradient elution.The heated electrospray ion source(HESI)was adopted to collect the data in positive ionization modes.The chemical constituents were identified and confirmed by analyzing the mass data from the reference substances and literature.A total of 62 isoquinoline alkaloids were identified in the methanol extract of Corydalis suaveolens Hance,including 15 berberine-type alkaloids,15 tetrahydro berberine-type alkaloids,14 benzylisoquinoline alkaloids,3 aporphine alkaloids,6 protamine type alkaloids,7 benzene not pyridine type alkaloids,and 2 dihydrobenzene not pyridine type alkaloids.This study comprehensively and systematically analyzed the components of isoquinoline alkaloids in the Coryda-lis suaveolens Hance,and provided a reference for the subsequent study of material basis and devel-opment.关键词
护心胆/UPLC-Q-Orbitrap-HRMS/异喹啉类生物碱/成分分析Key words
Corydalis suaveolens Hance/UPLC-Q-Orbitrap-HRMS/isoquinoline alkaloids/component analysis分类
化学化工引用本文复制引用
刘畅,周熙,罗辉泰,黄芳,吴惠勤..基于UPLC-Q-Orbitrap-HRMS技术鉴定护心胆中异喹啉类生物碱成分[J].分析测试学报,2023,42(12):1547-1557,11.基金项目
广东省科学院打造综合产业技术创新中心行动资金项目(2022GDASZH-2022010110) （2022GDASZH-2022010110）
