分析测试学报2023，Vol.42Issue(12)：1565-1572,8.DOI:10.19969/j.fxcsxb.23082202
超支化修饰亲水型阴离子色谱柱的制备与应用
Synthesis and Application of Hydrophilic Anion Chromatography Column Based on Hyperbranched Modification
摘要
Abstract
The employment of hyperbranched dendritic modification techniques,recognized for their versatile and precise structural customization,is pivotal in the hydrophobic tailoring of polymer-based stationary phases for chromatographic applications.This investigation utilized polystyrene-divi-nylbenzene microspheres as a foundational substrate and engaged 1,4-butanediol diglycidyl ether alongside tetraethylenepentamine as epoxy-amine grafting agents,with methylamine serving as the terminal capping agent,to synthesize a hydrophilic ion chromatography stationary phase.The devel-oped phase's morphology,structural attributes,and surface elemental composition were systemati-cally characterized employing techniques such as scanning electron microscopy(SEM),Brunauer Emmett Teller(BET),Fourier-transform infrared spectroscopy(FTIR),and X-ray photoelectron spectroscopy(XPS).Subsequent incorporation into a chromatographic column facilitated the assess-ment of its separation efficacy and retention dynamics for inorganic anions and weak organic acids.Parameters including separation degree and theoretical plate count for specified ions were quantitative-ly established.The retention characteristics and chromatographic resolution of the fabricated column were scrutinized under variable conditions of concentration,temperature,and eluent composition.Outcomes indicated that the integration of hydroxyl functionalities via epoxy-amine dendritic branch-ing markedly amplified the stationary phase's hydrophilicity.The retention of divalent anions,specif-ically sulfate(SO42-)and hydrogen phosphate(HPO42-),demonstrated a pronounced decrease,indi-cating minimal retention,while monovalent anions underwent ion exchange retention.Optimal sepa-ration of a suite of 12 anions,including fluoride(F-),acetate(CH3COO-),chlorite(ClO2-),bro-mate(BrO3-),chloride(Cl-),hydrogen phosphate(HPO42-),nitrite(NO2-),sulfate(SO42-),bro-mide(Br-),chlorate(ClO3-),oxalate(C2O42-),and nitrate(NO3-),was achieved within a 20 mins timeframe using a 3.6 mmol/L sodium carbonate(Na2CO3)eluent.Application of the tailored ion chromatography column to the detection of these anions in real samples,such as tap water,surface water,and industrial effluents,yielded a relative standard deviation(RSD,n=3)of less than 4.0%,underscoring its formidable potential for environmental analytical applications.关键词
离子色谱/超支化修饰/亲水型固定相/离子色谱柱/阴离子Key words
ion chromatography/hyperbranched modification/hydrophilic stationary phase/ion chromatographic column/anion分类
化学化工引用本文复制引用
蒙素仟,刘博,刘伟俊,刘瑞,桑柳波,赖华杰..超支化修饰亲水型阴离子色谱柱的制备与应用[J].分析测试学报,2023,42(12):1565-1572,8.基金项目
广州市重点研发计划(2023B03J1365) （2023B03J1365）
