分析测试学报2023，Vol.42Issue(12)：1598-1606,9.DOI:10.19969/j.fxcsxb.23062703
胶体金免疫层析法快速检测水产品中河豚毒素的研究
Rapid Detection of Tetrodotoxin in Aquatic Products by Colloidal Gold Immunochromatography Assay
摘要
Abstract
The aim of this paper is to establish a rapid detection method for tetrodotoxin by colloidal gold immunochromatography assay(GICA).Monoclonal antibody specific for tetrodotoxin was la-belled with colloidal gold as tracers.The effects of pH,antigen and antibody concentration,ion con-centration,surfactant type and sample pretreatment on the performance of GICA were carefully inves-tigated.The results showed that under the optimal conditions,the quantitative detection limit of the proposed assay for tetrodotoxin was 0.5 ng/mL,the linear range was 0.8-10.6 ng/mL,and the qual-itative detection limit was 12.0 ng/mL.The spiked recoveries for tetrodotoxin in pufferfish、nassari-idae samples ranged from 70.5%-110%with relative standard deviations of 3.7%-7.1%,and the re-sults were similar to those of the LC-MS/MS method.Compared with the traditional analytical tech-niques,the proposed GICA showed better feasibility,practicality and superiority for rapid detection in the field.关键词
河豚毒素/单克隆抗体/胶体金免疫层析/快速检测/水产品Key words
tetrodotoxin/monoclonal antibody/colloidal gold immunochromatography assay/rap-id detection/aquatic products分类
化学化工引用本文复制引用
韩金治,李亚茹,罗林,关甜,雷红涛,蔡伟谊,王炳志,徐振林..胶体金免疫层析法快速检测水产品中河豚毒素的研究[J].分析测试学报,2023,42(12):1598-1606,9.基金项目
国家自然科学基金(32061160473) （32061160473）
广东省海洋经济发展项目(GDNRC[2023]23) （GDNRC[2023]23）
