肝胆胰外科杂志2023，Vol.35Issue(12)：747-752,756,7.DOI:10.11952/j.issn.1007-1954.2023.12.010
搭载吉西他滨的SiO2@Fe3O4纳米材料抑制裸鼠胰腺癌移植瘤生长的实验研究
SiO2@Fe3O4 nanomaterials loaded with gemcitabine inhibit the growth of nude mice xenograft of pancreatic cancer:An experimental study
摘要
Abstract
Objective To investigate the efficacy and safety of SiO2@Fe3O4 nanomaterials loaded with gemcitabine(GEM)in the treatmen of pancreatic cancer.Methods FeCl3 nanoparticles were synthesized by the reaction of FeCl3,polyvinylpyrrolidone(PVP),and sodium acetate.These FeCl3 nanoparticles were then reacted with tetraethyl orthosilicate(TEOS)to obtain SiO2@Fe3O4 nanoparticles.The structure of the nanoparticles was characterized with transmission electron microscopy(TEM)and Fourier-transform infrared spectroscopy(FT-IR).GEM-SiO2@Fe3O4 nanoparticles were prepared by reacting GEM with the SiO2@Fe3O4 nanoparticles.The drug loading and entrapment efficiency of GEM-SiO2@Fe3O4 nanoparticles were calculated.In vitro drug release experiments were conducted to study the release profile of GEM from the nanoparticles.The anticancer effect of GEM-SiO2@Fe3O4 nanoparticles,organ damage,and pharmacokinetics were evaluated in nude mice xenograft of pancreatic cancer.Results TEM analysis revealed that the SiO2@Fe3O4 composite nanoparticles exhibited a spherical shape,ranging from 220 to 260 nm.FT-IR confirmed the successful synthesis of SiO2@Fe3O4 composite nanoparticles.The encapsulation efficiency of GEM-SiO2@Fe3O4 nanoparticles was determined to be 84.8%,with a drug loading of 7.8%.Experiments on nude mice xenograft of pancreatic cancer demonstrated that GEM-SiO2@Fe3O4 composite nanoparticles exhibited superior anti-tumor effects than that of free GEM.Furthermore,these nanoparticles did not cause significant damage to normal liver,heart,spleen,lungs,or kidney tissues.Conclusion This experimental study shows that SiO2@Fe3O4 nanomaterials loaded with GEM effectively inhibit the growth of nude mice xenograft of pancreatic cancer,without significant damage in normal tissues.关键词
胰腺癌/吉西他滨/磁性纳米复合材料/裸鼠/实验研究Key words
pancreatic cancer/gemcitabine/magnetic nanocomposites/nude mice/experimental study分类
临床医学引用本文复制引用
全棋,管时伟,林泉,彭明晖,俞海波..搭载吉西他滨的SiO2@Fe3O4纳米材料抑制裸鼠胰腺癌移植瘤生长的实验研究[J].肝胆胰外科杂志,2023,35(12):747-752,756,7.基金项目
浙江省医药卫生科技计划项目(2020RC113,2022KY1202). （2020RC113,2022KY1202）
