广西林业科学2023，Vol.52Issue(6)：726-732,7.DOI:10.19692/j.issn.1006-1126.20230608
基于ISSR分子标记技术的土沉香遗传多样性分析
Analysis on Genetic Diversity of Aquilaria sinensis Based on ISSR Molecular Markers
摘要
Abstract
In order to effectively protect and utilize genetic diversity of Aquilaria sinensis,taking 147 families from 13 populations of A.sinensis in Guangxi,Hainan and Yunnan as materials,polymorphism analysis and ge-netic diversity comparison were carried out using Inter-Simple Sequence Repeat(ISSR)molecular markers and POPGEN 32 software,clustering analysis was carried out by NTSYS software,and correlations among genetic distances and geographic distances were analyzed using Mantle test.Results showed that 291 loci were ampli-fied by 34 pairs of ISSR primers,including 230 polymorphic loci.Average percentage of polymorphic loci was 78.97%.Mean of Nei's gene diversity index was 0.226 1,mean of Shannon's information index was 0.332 0,mean of genetic differentiation coefficient was 0.265 0,and mean of gene flow was 1.387 1,which indicated that there were relative high genetic diversity and extremely high genetic differentiation in 13 populations of A.si-nensis.The smallest genetic distance and the greatest genetic consistency were found between Hainan Tun-chang and Hainan Chengmai populations,and the largest genetic distance and the smallest genetic consistency were found between Hainan Ledong and Guangxi Pubei populations.When genetic distance of 0.90 was used as threshold,13 populations of A.sinensis could be divided into 3 major categories.Explanatory rate of genetic dis-tance and geographical distance was 3.1%,showing no correlation.关键词
ISSR/遗传多样性/聚类分析/土沉香Key words
ISSR/genetic diversity/clustering analysis/Aquilaria sinensis分类
农业科学引用本文复制引用
于彤,申文辉,覃日亮,韦文龙,李俊晓,谭长强,滕维超,曹艳云,郝海坤..基于ISSR分子标记技术的土沉香遗传多样性分析[J].广西林业科学,2023,52(6):726-732,7.基金项目
国家重点研发计划课题(2017YFD0601101,2016YFD0600606) （2017YFD0601101,2016YFD0600606）
广西优良用材林资源培育重点实验室自主课题(20-A-02-02) （20-A-02-02）
广西优良用材林资源培育重点实验室开发课题(2020B-02-01) （2020B-02-01）
