南方农业学报2023，Vol.54Issue(8)：2207-2217,11.DOI:10.3969/j.issn.2095-1191.2023.08.002
不同氮素形态配比对烟草青枯病发生及土壤细菌群落的影响
Effects of different nitrogen form ratios on the occurrence of tobacco bacterial wilt and soil bacterial community
摘要
Abstract
[Objective]The purpose of the study was to explore the relationship between different nitrogen form ratios and the occurrence of tobacco bacterial wilt,the physical and chemical properties of tobacco rhizosphere soil and soil bac-terial communities,and to screen out the key microorganisms and soil factors,so as to provide theoretical basis for the pre-vention and control of tobacco bacterial wilt and the rational application of nitrogen fertilizers.[Method]Using plots with severe occurrence of tobacco bacterial wilt in previous years as experimental plots,five different nitrogen form ratios and conventional control(CK)treatments were designed to investigate the occurrence of tobacco bacterial wilt in different treatments with different nitrate nitrogen and ammonium nitrogen ratios.The physical and chemical properties of the rhizo-sphere soil were determined by collecting the rhizosphere soil of tobacco plants treated with different nitrate nitrogen and ammonium nitrogen ratios.Using 16S rDNA sequencing technology,the differences in the occurrence of tobacco bacterial wilt and the bacterial community structure in rhizosphere soil under different nitrate nitrogen and ammonium nitrogen ratios were investigated.[Result]Compared with CK treatment,when nitrate nitrogen and ammonium nitrogen were ap-plied in combination at a ratio of 1∶1,the morbidity and disease index of tobacco bacterial wilt were effectively reduced,and the SPAD value of tobacco leaf was increased.When nitrate nitrogen and ammonium nitrogen were applied in combi-nation at a ratio of 7∶3,the abundance and evenness of soil bacterial community were increased.The results of PCoA ana-lysis indicated that different nitrogen form ratios had significant effects on soil bacterial communities(P<0.05,the same below).At the phyla classification level,Proteobacteria and Acidobacteria were the most dominant soil bacteria phyla.At the genus classification level,Bacillus was the most dominant genus of soil bacteria,followed by Sphingomonas.,Candi-datus_Udaeobacter,Bryobacter and Flavobacterium.The results of redundancy analysis(RDA)and Pearson analysis in-dicated that soil organic matter(SOM),available potassium(AK),and nitrate nitrogen(NO-N)were the main physical and chemical factors affecting the occurrence of changes in soil bacterial communities.[Conclusion]Different nitrogen form ratios can affect the occurrence of tobacco bacterial wilt,as well as the diversity and species composition of soil bac-terial communities.The ratio of nitrate nitrogen to ammonium nitrogenat 1∶1 is the optimal treatment,which can effec-tively reduce the morbidity and disease index of tobacco bacterial wilt and increase the relative abundance of Bacillus and Flavobacterium in the soil.Properly increasing the proportion of nitrate nitrogen is beneficial for the construction of soil healthy microecology.关键词
硝态氮/铵态氮/烟草青枯病/土壤/细菌群落Key words
nitrate nitrogen/ammonium nitrogen/tobacco bacterial wilt/soil/bacterial community分类
农业科技引用本文复制引用
宝童曦,吕芬,高正锋,徐丝,李先伟,黄荣斌,邹应啟,周庆兰,吕敏,白羽祥..不同氮素形态配比对烟草青枯病发生及土壤细菌群落的影响[J].南方农业学报,2023,54(8):2207-2217,11.基金项目
国家自然科学基金项目(32160517) （32160517）
贵州省烟草公司重点研发项目(2021XM15) National Natural Science Foundation of China(32160517) （2021XM15）
Key Research and Development Pro-ject of Guizhou Tobacco Company(2021XM15) （2021XM15）
