南方农业学报2023，Vol.54Issue(8)：2236-2244,9.DOI:10.3969/j.issn.2095-1191.2023.08.005
不同pH和镉浓度对雪茄烟种子萌发和幼苗生长生理的影响
Effects of different pH and cadmium concentrations on physio-logy of seed germination and seedling growth of cigar
摘要
Abstract
[Objective]This paper explored the effect of different pH and cadmium(Cd)concentrations on physiology of seed germination and seedling growth of cigar,in order to provide reference for obtaining high-quality and safe cigar raw material.[Method]Hydroponics experiment was carried out and different pH(3,5,7,9 and 11)and Cd concentra-tions(0,10,50 and 250µmol/L)were set.The seed germination indexes(germination rate and germination energy),seedling growth physiological indexes[radicle length,embryo length,dry matter weight,chlorophyll content,nitrate re-ductase activity,malondialdehyde(MDA)content,root activity]and Cd content in various parts of seedlings were mea-sured.[Result]Under the same Cd concentration,the germination rate of cigar seeds first increased and then decreased with the increase of pH.Under the same pH,the germination rate of cigar seeds treated with Cd decreased gradually with the increase of Cd concentration.The germination energy and germination rate of seeds were the highest when pH was 7 and the Cd concentration was 10µmol/L.Under the same pH,the radicle length of cigar seedlings first increased and then decreased with the increase of Cd concentration.The embryo length of seedlings decreased with the increase of Cd concen-tration when pH was 3 and 7.Under other pH conditions,the embryo length of seedlings increased at first and then de-creased with the increase of Cd concentration.The radicle length and embryo length reached the longest when pH was 9 and Cd concentration was 10 umol/L.Except for the treatment of 0µmol/L Cd,the dry matter weight of cigar seedlings decreased with the increase of Cd concentration,and increased at first and then decreased with the increase of pH.Under the treatment of 10-250µmol/L Cd,the Cd content in roots,stems and leaves of cigar seedlings decreased gradually with the increase of pH,and increased with the increase of Cd concentration.The chlorophyll content,nitrate reductase activi-ty and root activity of cigar seedlings increased at first and then decreased with the increase of pH,but it decreased with the increase of Cd concentration.The content of MDA in cigar seedlings increased with the increase of Cd concentration when pH was 5-11.Under the treatment of 10-250µmol/L Cd,the content of MDA increased at first,then decreased and then increased with the increase of pH,and reached the minimum when pH was 7.[Conclusion]Low Cd concentration promotes the germination of cigar seeds,but the growth physiology of cigar seedlings is inhibited with the increase of Cd concentration.The change of pH will affect the absorption and accumulation of heavy metal Cd by cigar seedlings,and then affect the normal growth and dry matter accumulation of cigar seedlings.When pH is 7,10µmol/L Cd treatment in-creases the chlorophyll content,nitrate reductase activity and root activity of cigar seedlings,being beneficial to the growth of cigar seedlings.关键词
雪茄烟/pH/镉/种子萌发/幼苗生长生理Key words
cigar/pH/cadmium/seed germination/seedling growth physiology分类
农业科技引用本文复制引用
贺远,伍宗毅,陈国瑀,高华军,李龙科,刘海伟,刘春奎,陈耘丰..不同pH和镉浓度对雪茄烟种子萌发和幼苗生长生理的影响[J].南方农业学报,2023,54(8):2236-2244,9.基金项目
河南省科技攻关项目(232102230107) （232102230107）
中国烟草总公司科技重大专项[110202101013(XJ-05)] Henan Science and Technology Key Project(232102230107) （XJ-05）
Science and Technology Key Project of China National Tobacco Corporation[110202101013(XJ-05)] （XJ-05）
