南方农业学报2023，Vol.54Issue(8)：2320-2329,10.DOI:10.3969/j.issn.2095-1191.2023.08.013
桑树热激转录因子(Hsf)基因家族克隆及MnHsfA1基因表达模式分析
Cloning of heat shock transcription factor(Hsf)gene family from mulberry and analysis of MnHsfA1 gene expression pattern
摘要
Abstract
[Objective]This paper cloned the heat shock transcription factor(Hsf)gene family members in mulberry,analyzed expression pattern of the MnHsfA1 genes to provide theoretical reference for in-depth research on the regulatory mechanism of this family in mulberry heat stress response and mulberry breeding.[Method]Using seedlings of Morus notabilis as experimental materials,the MnHsfs genes were cloned.And the physicochemical properties,conserved domains,signal peptides,transmembrane domains,subcellular localization,and promoter cis acting elements of MnHsfs proteins were predicted by multiple sequence alignment,phylogenetic tree construction,and MEME conserved motif.The expression pattern of MnHsfA1 genes under high temperature and drought stress were analyzed using real-time fluo-rescence quantitative PCR.[Result]Fourteen full-length coding sequences of MnHsfs genes were cloned from mulberry trees,including MnHsfA4a,MnHsfA4b,MnHsfA2,MnHsfA5,MnHsfA6b-1,MnHsfA6b-2,MnHsfB4-2,MnHsfA8,MnHsfB3,MnHsfB2b,MnHsfC1,MnHsfB2a,MnHsfB4-1 and MnHsfA1.Their open reading frame lengths were 1239,1278,1404,1431,1101,1140,1185,1197,711,996,1029,936,900 and 1437 bp and encoded 412,425,467,476,366,379,394,398,236,331,342,312,299 and 478 amino acids residues respectively.And most of MnHsfs proteins were hydrophilic and unstable acidic proteins.MnHsfs protein included domains such as function domain(DBD),oligomeriza-tion function domain(OD),AHA,and KLFGV,and were divided into class A,class B,and class C.The similarity between MnHsfA1 and HsfA1 of apple,spinach,and Arabidopsis were the highest.Compared with the control,the expression of MnHsfA1 gene and mRNA transcription level were significantly up regulated under high temperature and drought stress treatments(P<0.05).[Conclusion]MnHsfs genes have relatively high genetic conservatism,and the expression level of MnHsfA1 gene is up regulated under high temperature and drought stress.The cis acting element of MnHsfA1 gene pro-moter contains hormone responsive elements such as abscisic acid,methyl jasmonate,gibberellin,as well as transcrip-tion factors related to various abiotic stresses.It is speculated that it may regulate multiple abiotic stresses simultaneously.关键词
桑树/热激转录因子(Hsf)/系统发育进化树/胁迫/顺式作用元件Key words
mulberry/heat shock transcription factor(Hsf)/phylogenetic tree/stress/cis acting element分类
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刘丹,林强,邱长玉,韦伟,陆晓媚,黄胜,曾燕蓉,朱光书,张朝华,何骥..桑树热激转录因子(Hsf)基因家族克隆及MnHsfA1基因表达模式分析[J].南方农业学报,2023,54(8):2320-2329,10.基金项目
国家重点研发计划项目(2022YFD1601301) （2022YFD1601301）
国家现代农业产业技术体系建设专项(CARS-18) National Key Research and Development Program of China(2022YFD1601301) （CARS-18）
China Agricul-ture Research System(CARS-18) （CARS-18）
