南方农业学报2023,Vol.54Issue(8):2388-2395,8.DOI:10.3969/j.issn.2095-1191.2023.08.020
8种海南热带水果的抗氧化活性比较分析
Antioxidant activity comparison of eight kinds of tropical fruits in Hainan
摘要
Abstract
[Objective]The antioxidant activity and the contents of total phenolics and flavonoids of eight kinds of tropical fruits in Hainan were determined,and the differences of antioxidant activity and antioxidant substances in eight kinds of fruits were compared,so as to provide certain nutritional reference value for people to eat tropical fruits,it also provided reference for the development and utilization of tropical fruit related products with strong antioxidant activity.[Method]Eight tropical fruits including Manilkara zapota,Syzygium samarangense,Garcinia mangostana,Durio zibet-hinus,Lucuma nervosa,Passiflora edulis,Annona squamosa and Artocarpus heterophyllus were studied in this experi-ment.The antioxidant activity of the samples was determined by 1,1-dipheny1-2-picrylhydrazyl(DPPH)free radical method,ferric reducing antioxidant power(FRAP)and diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation free radical scavenging activity method.The total phenolics content was determined by the Folin-Ciocalteu method,and the total flavonoids content was determined by AlCl3 chromotest method.The correlation analysis of three different determination methods and two antioxidant substances were carried out,and the results of antioxidant activity were analyzed by technique for order preference by similarity to ideal solution(TOPSIS)method.[Result]The most effective antioxidant activity measured by DPPH method and FRAP method was L.nervosa(178.62 and 140.83 μmol Trolox/g),and that of M.zapota was the weakest.The highest antioxidant activity was found in G.mangostana(157.06 μmol Trolox/g)and that of M.zapota was the weakest by ABTS method.At the same time,the content of total phenolics(3.80%)and total flavonoids(7.60%)in L.nervosa was the highest among the eight kinds of fruits,the con-tent of total phenolics in M.zapota was the lowest(0.09%),and the content of total flavonoids in D.zibethinus was the lowest(1.07%).Correlation analysis of three different determination methods and two antioxidant substances was found,the correlation coefficient between DPPH method and total phenolics and total flavonoids contents reached more than 0.81,the correlation between FRAP method and total phenolics and total flavonoids contents was more than 0.90.The re-sults were analyzed by the TOPSIS method,and the antioxidant activity was ranked as:L.nervosa>G.mangostana>S.sa-marangense>P.edulis>A.squamosa>A.heterophyllus.>D.zibethinus>M.zapota.[Conclusion]All the eight kinds of tropical fruits have antioxidant activity,the antioxidant activity of L.nervosa is strong,and that of M.zapota is weak.关键词
热带水果/抗氧化活性/总酚/总黄酮/TOPSIS法/海南Key words
tropical fruit/antioxidant activity/total phenolics/total flavonoids/TOPSIS method/Hainan分类
园艺学与植物营养学引用本文复制引用
吕金慧,郑中兵,吴涛,孙娅辉,陈萍..8种海南热带水果的抗氧化活性比较分析[J].南方农业学报,2023,54(8):2388-2395,8.基金项目
海南省自然科学基金项目(320RC491) Hainan Natural Science Foundation(320RC491) (320RC491)