南方农业学报2023，Vol.54Issue(8)：2436-2443,8.DOI:10.3969/j.issn.2095-1191.2023.08.025
江西东方蜜蜂微卫星DNA的遗传多样性分析
Genetic diversity analysis of microsatellite DNA of Apis cerana in Jiangxi
摘要
Abstract
[Objective]To clarify the level of genetic diversity and differentiation regular patterns of Apis cerana un-der habitat conditions in Jiangxi,and provide theoretical basis for promoting the protection and utilization of the genetic resources of A.cerana in Jiangxi,as well as the protection and rational layout of A.cerana genetic resources in China.[Method]A total of 37 microsatellite markers were selected to analyze the genetic characteristics,genetic diversity and genetic differentiation of 704 populations of A.cerana from 10 representative sites in Jiangxi.Excel Microsatellite Toolkit 3.1 was used to calculate the observed heterozygosity(Ho),expected heterozygosity(He),number of alleles(Na)and polymorphic information content(PIC).PopGene 1.31 was used to calculate the effective allele number(Ne)and Shan-non index(I),and GenAlEx 6.5 and R 4.0.3 were used to carry out principal coordinate analysis(PCoA)and principal component discriminant analysis(DAPC)respectively.Genetic differentiation index(Fst)between sample sites was ana-lyzed using Genepop on Web online software.Gene flow(Nm)was calculated using Fst=1/(1+4Nm),and UPGMA den-drogram was constructed using POPTREE2.[Result]A total of 374 alleles were detected from 704 populations of A.cera-na at 10 sampling sites in Jiangxi using 37 microsatellite markers.Among them,21 microsatellite markers(AP243,BI278,AP249,AT185,BI225,AC139,Ap313,AT004,AC045,K0715,AC011,AP189,BI314,Ap085,AT101,244T,Ac-1,Ac-2,Ac-5,Ac-26 and Ac-35)of PIC were higher than the average.The Na values of 10 sample sites of A.cerana populations in Jiangxi ranged from 5.2857 to 6.3125,with an average value of 5.8247;Ho ranged from 0.3951 to 0.4602,with an average value of 0.4197;He ranged from 0.4211 to 0.4676,with an average value of 0.4437;Ne ranged from 2.7991 to 3.2596,with an average value of 2.9205;PIC ranged from 0.3909 to 0.4366,with an average value of 0.4133;I ranged from 0.9090 to 1.0106,with an average value of 0.9542;inbreeding coefficient(Fis)between 10 sample sites of A.cerana ranged from-0.036 to 0.091,Fst ranged from 0.0059 to 0.0294,and Nm ranged from 8.2534 to 42.1229.PCoA analysis,DAPC analysis and UPGMA dendrogram all showed that the population genetic differentiation of A.cerana in Jiangxi has not yet occurred.[Conclusion]The genetic diversity of A.cerana in Jiangxi is at a moderate level nation-wide,and no population genetic differentiation has been found.Moreover,the role of gene exchange in the genetic dif-ferentiation process of A.ceranais greater than that of environmental selection.关键词
东方蜜蜂/微卫星/遗传多样性/遗传分化/江西Key words
Apis cerana/microsatellite/genetic diversity/genetic differentiation/Jiangxi分类
农业科技引用本文复制引用
朱诗谣,周姝婧,朱翔杰,徐新建,苏显冰,张芷宁,姜荃耀,周冰峰..江西东方蜜蜂微卫星DNA的遗传多样性分析[J].南方农业学报,2023,54(8):2436-2443,8.基金项目
国家自然科学青年基金项目(31902218) （31902218）
国家蜂产业技术体系建设专项(CARS-45-KXJ11) （CARS-45-KXJ11）
福建省大学生创新创业训练计划项目(X202210359177) Youth Foundation of National Natural Science of China(31902218) （X202210359177）
China Agriculture Research System—Bee(CARS-45-KXJ11) （CARS-45-KXJ11）
Fujian Province College Students Innovation and Entrepreneurship Training Project(X202210359177) （X202210359177）
