广西医学2023，Vol.45Issue(19)：2361-2367,2373,8.DOI:10.11675/j.issn.0253-4304.2023.19.12
益肺宣肺降浊方对星形胶质细胞-神经元氧糖剥夺损伤的缓解作用及其可能机制
Effect of Yifei Xuanfei Jiangzhuo Prescription for alleviating astrocytes-neuronal oxygen glucose deprivation damage and its possible mechanism
摘要
Abstract
Objective To analyze the effect of Yifei Xuanfei Jiangzhuo Prescription for alleviating astrocytes-neuronal oxygen glucose deprivation(OGD)damage,and to explore its possible mechanism based on choline acetyltransferase(ChAT),α7-nicotinic acetylcholine receptor(α7-nAChR),and inflammatory factors.Methods(1)Control serum and drug-contained serum of Yifei Xuanfei Jiangzhuo Prescription were prepared.(2)Hippocampal astrocytes and neurons were isolated and cultured from neonatal rats.Cells were assigned to control group,OGD group,OGD +YFXF group,or OGD +YFXF +anisodamine group.In the control group,primary hippocampal neurons and astrocytes were routinely cultured;furthermore,in the OGD,OGD + YFXF,and OGD + YFXF + anisodamine groups,astrocytes-neuronal OGD model was established,and high glucose DMEM of 5%control serum,high glucose DMEM of 5%Yifei Xuanfei Jiangzhuo Prescription with drug-contained serum,and high glucose DMEM of 5%Yifei Xuanfei Jiangzhuo Prescription with drug-contained serum and 100 μg/mL anisodamine were used to culture,respectively.(3)After 24 hours of intervention,cell morphology was observed in various groups.The expressions of astrocytes and neurons markers,and mRNA and protein expressions of content of reactive oxygen species,ChAT activity,α7-nAChR,and ChAT in astrocytes,as well as supernate lactate dehydrogenase(LDH),interleukin(IL)-1β,tumor necrosis factor α(TNF-α),and IL-6 levels were detected.Results Compared with the control group,the OGD group yielded weakened fluorescence intensity of astrocytes and neurons markers,and presented as abnormal neuronal morphology,increased content of reactive oxygen species of astrocytes,elevated supernate LDH,IL-1β,TNF-α,and IL-6 levels,decreased ChAT activity of astrocytes,and down-regulated mRNA and protein expressions of α7-nAChR and ChAT of astrocytes(P<0.05).Compared with the OGD group,the OGD+YFXF group exhibited enhanced fluorescence intensity of astrocytes and neurons markers,and presented as improved neuronal morphology,decreased content of reactive oxygen species of astrocytes and supernate LDH,IL-1β,TNF-α,and IL-6 levels,enhanced ChAT activity of astrocytes,and up-regulated mRNA and protein expressions of α7-nAChR and ChAT of astrocytes(P<0.05).Compared with the OGD +YFXF group,the OGD +YFXF +anisodamine group interpreted weakened fluorescence intensity of astrocytes and neurons markers,and presented as abnormal neuronal morphology,increased content of reactive oxygen species of astrocytes and supernate LDH and TNF-α levels,and down-regulated mRNA and protein expressions of α7-nAChR of astrocytes(P<0.05).Conclusion Yifei Xuanfei Jiangzhuo Prescription may alleviate astrocytes-neuronal OGD damage through up-regulating α7-nAChR expression,enhancing ChAT activity,inhibiting inflammatory responses in hippocampal astrocytes,and it may be the mechanism of this prescription for ameliorating vascular dementia mediated by ischemia reperfusion injury.关键词
益肺宣肺降浊方/星形胶质细胞/神经元/氧糖剥夺/血管性痴呆/α7烟碱型乙酰胆碱受体/胆碱乙酰转移酶/炎症因子Key words
Yifei Xuanfei Jiangzhuo Prescription/Astrocytes/Neurons/Oxygen glucose deprivation/Vascular dementia/α7-nicotinic acetylcholine receptor/Choline acetyltransferase/Inflammatory factors分类
医药卫生引用本文复制引用
朱健敏,陈炜,胡跃强,蒋凌飞,吴林..益肺宣肺降浊方对星形胶质细胞-神经元氧糖剥夺损伤的缓解作用及其可能机制[J].广西医学,2023,45(19):2361-2367,2373,8.基金项目
国家自然科学基金(82060844,82160885) （82060844,82160885）
广西中医药重点学科建设项目(GZXK-Z-20-13) （GZXK-Z-20-13）
广西中医药大学研究生教育创新计划项目(YCBXJ2022023) （YCBXJ2022023）
广西中医基础研究重点实验室课题(17-259-49-01) （17-259-49-01）
