广西医学2023，Vol.45Issue(19)：2368-2373,6.DOI:10.11675/j.issn.0253-4304.2023.19.13
芒柄花黄素对鼻咽癌细胞增殖和周期的影响及其可能机制
Effect of formononetin on proliferation and cycle of nasopharyngeal cancerous cells and its possible mechanism
摘要
Abstract
Objective To investigate the effect of formononetin on proliferation and cycle of nasopharyngeal carcinoma 5-8F cells and its possible mechanism.Methods Cells of 5-8F were assigned to solution control group(adding complete culture solution),formononetin in different concentrations groups(adding formononetin with final concentration of 10 μmol/L,20 μmol/L,40 μmol/L,80 μmol/L,and 160 μmol/L),or 5-fluorouracil(5-Fu)group(adding 5-Fu).After 24 and 48 hours of respective intervention,the MTT method was used to detect the proliferation rate of 5-8F cells in various groups.After 24 hours of intervention,the PI staining was employed to detect 5-8F cells cycle in the solution control group,20 μmol/L formononetin group,40 μmol/L formononetin group,80 μmol/L formononetin group,and 5-Fu group.After 24 hours of intervention,the Western blot was used to detect protein expressions of 5-8F cells proliferating cell nuclear antigen(PCNA)and cyclin dependent kinase 1(CDK1)in the solution control group,20 μmol/L formononetin group,40 μmol/L formononetin group,and5-Fu group.Results(1)After24 and48 hours of intervention,the 20 μmol/L,40 μmol/L,80 μmol/L,and 160 μmol/L formononetin groups and 5-Fu group exhibited lower proliferation rates of 5-8F cells as compared with the solution control group(P<0.05).After24 hours of intervention,the proliferation rates of 5-8F cells in the 10 μmol/L,20 μmol/L,40 μmol/L formononetin groups were higher than those in the 5-Fu group.After 48 hours of intervention,the proliferation rates of 5-8F cells in the formononetin with various concentrations groups were higher than those in the 5-Fu group(P<0.05).(2)Compared with the solution control group,the proportion of cells in G0/G1 phase was decreased,while proportion of cells in G2/M phase was increased in the 20 μmol/L,40 μmol/L,and 80 μmol/L formononetin groups and the 5-Fu group(P<0.05).The proportion of cells in G0/G1 phase in the 20 μmol/L,40 μmol/L,and 80 μmol/L formononetin groups was higher than that in the 5-Fu group,whereas the proportion of cells in G2/M phase was lower than that in the 5-Fu group(P<0.05).(3)Compared with the solution control group,the protein expressions of CDK1 and PCNA were decreased in the 20 μmol/L and 40 μmol/L formononetin groups and the 5-Fu group(P<0.05);moreover,the 5-Fu group exhibited higher protein expressions of CDK1 and PCNA as compared with the 40 μmol/L formononetin group,and a lower PCNA protein expression as compared with the 20 μmol/L formononetin group(P<0.05).Conclusion Formononetin is able to inhibit proliferation of nasopharyngeal carcinoma 5-8F cells,and the cell cycle is blocked in G2/M phase.Its possible mechanism may be related to the down-regulated expressions of PCNA and CDK1;however,the anti-tumor mechanism of drug is complex,the anti-nasopharyngeal carcinoma effect of formononetin remains to be optimized,and its specific mechanism remains to be further explored.关键词
鼻咽癌/芒柄花黄素/细胞增殖/细胞周期/增殖细胞核抗原/细胞周期蛋白依赖性激酶1Key words
Nasopharyngeal carcinoma/Formononetin/Cell proliferation/Cell cycle/Proliferating cell nuclear antigen/Cyclin dependent kinase 1分类
医药卫生引用本文复制引用
湛芳,贺诗雅,孙梦,赵心瑶,胡熙苒,史红健..芒柄花黄素对鼻咽癌细胞增殖和周期的影响及其可能机制[J].广西医学,2023,45(19):2368-2373,6.基金项目
湖南省中医药科研计划项目(D2022105) （D2022105）
湖南省教育厅科学研究项目(21C0241) （21C0241）
湖南省大学生创新创业训练计划项目(20212461) （20212461）
湖南中医药大学校级科研项目(2021XJJJ008) （2021XJJJ008）
