工业微生物2023，Vol.53Issue(6)：41-44,4.DOI:10.3969/j.issn.1001-6678.2023.06.014
基于骨髓间充质干细胞分离培养的原代细胞培养技术在细胞生物学实验课程中的应用和思考
The Application and Thinking of Primary Cell Culture based on Isolation and Culture of Bone Marrow Mesenchymal Stem Cells from Mice in Cell Biology Experiment
摘要
Abstract
Cell is the basic structural and functional unit of life activities,and it is indispensable for carrying out various researches in cell biology.Cell culture is the basis and premise of many cell biology researches,and it is also one of the most basic experimental operation techniques in biology.Primary cell culture is the main method to obtain cells.Incorporating the primary culture technology based on bone marrow mesenchymal stem cell isolation and culture into the cell biology experiment course can enrich the experimental course content,improve the course construction,and comprehensively promote the cell culture ability of students.The implementation of this experiment has a good consolidation effect on the students'research ability of cell culture.Moreover,the introduction of scientific research content into experimental teaching can strengthen students'understanding of the multi-potential differentiation of bone marrow mesenchymal stem cells,stimulate students'multi-experimental design thinking and the motivation to carry out comprehensive innovative experiments.关键词
骨髓间充质干细胞/原代细胞培养/细胞培养/细胞生物学实验/无菌操作Key words
bone marrow mesenchymal stem cells/primary cell culture/cell culture/cell biology experiment/aseptic operation引用本文复制引用
吕欢欢,刘悦彤,王建萍,吕毅..基于骨髓间充质干细胞分离培养的原代细胞培养技术在细胞生物学实验课程中的应用和思考[J].工业微生物,2023,53(6):41-44,4.基金项目
2023年西北工业大学课程建设项目"细胞生物学实验(英)"(PX-27232098). （英）
