工业微生物2023，Vol.53Issue(6)：160-162,3.DOI:10.3969/j.issn.1001-6678.2023.06.043
1-脱氧-D-木桐糖-5-磷酸合酶(DXS)对枯草芽孢杆菌代谢途径的影响
Effect of 1-deoxy-D-xylose 5-phosphate synthase(DXS)on the Metabolic Pathway of Bacillus subtilis
李雁洁 1韩旭2
作者信息
- 1. 苏州健雄职业技术学院,江苏苏州 215411
- 2. 沈阳化工大学,辽宁 沈阳 110142
- 折叠
摘要
Abstract
1-deoxy-D-xylulose-5-phosphate synthase(DXS)is an essential enzyme in the biosynthesis pathway of terpenoids,namely the 2-C-methyl-Derythritol 4-phosphate(MEP)pathway.This enzyme catalyzes the first step reaction of the MEP pathway,condensing pyruvate and glyceraldehyde-3-phosphate to form 1-deoxy-D-xylose 5-phosphate(DXP).Bacillus subtilis has a clear genetic background and is an excellent potential terpene producing bacterium.However,research on the key enzyme DXS in the MEP pathway of Bacillus subtilis is still insufficient.In this study the dxs gene of Bacillus subtilis was cloned,and a recombinant expression plasmid for this genewas also constructed.The recombinant expression plasmid was transferred into Bacillus subtilis for overexpression,and the effect of DXS on the MEP metabolic pathway in Bacillus subtilis was analyzed..The results showed that overexpression of the dxs gene caused abnormal metabolic pathways and increased carbon dioxide content in Bacillus subtilis.关键词
DXS/MEP/枯草芽孢杆菌Key words
DXS/MEP/Bacillus subtilis引用本文复制引用
李雁洁,韩旭..1-脱氧-D-木桐糖-5-磷酸合酶(DXS)对枯草芽孢杆菌代谢途径的影响[J].工业微生物,2023,53(6):160-162,3.
