海南师范大学学报（自然科学版）2023，Vol.36Issue(4)：420-428,9.DOI:10.12051/j.issn.1674-4942.2023.04.010
红榄李来源内生真菌Penicillium oxalicum HLLG-13次级代谢产物与生物活性研究
Study on the Secondary Metabolites and Biological Activity from Lumnitzera littorea-Derived Endophytic Fungus Penicillium oxalicum HLLG-13
摘要
Abstract
Twenty-one compounds were isolated from the EtOAc extract of the fermentation broth of the Lumnitzera litto-rea-derived fungus Penicillium oxalicum HLLG-13.Their structures were elucidated by 1D NMR,MS spectral analyses and compared with literature data.The compounds were identified as 2-(4-hydroxyphenyl)ethyl(2S)-2-hydroxypropanoate(1),2-(4-hydroxyphenyl)ethylacetate(2),methyl(4-hydroxyphenyl)acetate(3),4-hydroxybenzeneethanol(4),2,4,5-tri-methylresorcinol(5),1-(2,6-dihydroxyphenyl)butan-1-one(6),rosmarinosin C(7),penicyclone A(8),penicyclone C(9),2-(N-acetylamino)phenol(10),N-acetyltyramine(11),2-[[(2S)-2-hydroxy-1-oxopropyl]amino]benzamide(12),peniami-done A(13),meleagrin(14),7-hydroxy-2-(2'S-hydroxypropyl)-5-methylchromone(15),6,8-dihydroxy-3-methylisocou-marin(16),(-)-6-hydroxymellein(17),(3R)-5-chloro-3,4-dihydro-6,8-dihydroxy-3-methyl-1H-2-benzopyran-1-one(18),(3R)-3,4-dihydro-6,8-dihydroxy-7-methoxy-3-methyl-1H-2-benzopyran-1-one(19),(-)-citreoisocoumarinol(20)and de-O-methyldiaporthin(21).All compounds exhibited no antibacterial activity against the five human pathogenic bacterium and three vibrios.Compound 16 showed moderate neuroprotective activity,which could increase the cell survival rate of H2O2 induced oxidative damage HT22 cells from 43.36%to 60.15%at the concentration of 200 μmol/L.关键词
红榄李/Penicillium oxalicum/次级代谢产物/抗菌活性/神经保护活性Key words
Lumnitzera littorea/Penicillium oxalicum/secondary metabolites/antibacterial activity/naeuroprotective activity分类
医药卫生引用本文复制引用
王越,张玉琴,徐喆菲,白琪琪,陈文豪,周学明,陈光英..红榄李来源内生真菌Penicillium oxalicum HLLG-13次级代谢产物与生物活性研究[J].海南师范大学学报（自然科学版）,2023,36(4):420-428,9.基金项目
国家自然科学基金项目(22177023,41866005) （22177023,41866005）
海南省自然科学基金项目(221RC1054) （221RC1054）
海南省重大科技计划项目(ZDKJ202008) （ZDKJ202008）
中央引导地方科技发展资金项目(ZY2022HN08) （ZY2022HN08）
