经HIV包膜蛋白gp120刺激的T细胞外泌体促进巨噬细胞M2极化
Extracellular vesicles from T cells stimulated by HIV envelope protein gp120 promote macrophage M2 polarization
摘要
Abstract
Objective:This study aimed to investigate the effects of exosomes from human T lymphocyte line(H9)treated with HIV envelope protein gp120 on macrophage polarization.Methods:The viability of gp120-treated H9 T lymphocytes was as-sessed using the CCK8 assay.Inflammatory cytokine levels in the supernatant of H9 cells were determined by ELISA.Exosome characteristics were identified through electron microscopy and Western blot experiments.PHK67 staining was employed to ob-serve the uptake of exosomes by macrophages.Finally,macrophage polarization markers were detected using ELISA and immuno-fluorescence to analyze the impact of gp120-treated T cell exosomes on macrophage polarization.Results:HIV gp120 protein in-hibited the proliferation of human T lymphocytes H9 and promoted the release of inflammatory cytokines.Exosomes from H9 T lymphocytes were successfully isolated,displaying a cup-shaped membranous structure under electron microscopy and overex-pressing marker proteins CD9,CD63,and CD81.PHK67 staining results indicated that exosomes from H9 cells could be internal-ized by macrophages.Exosomes from gp120-treated H9 cells promoted the polarization of macrophages towards the M2 pheno-type.Conclusion:Exosomes secreted by human T lymphocytes H9 treated with HIV envelope protein gp120 can promote M2 po-larization of macrophages,suggesting a potential novel mechanism of gp120 in immune modulation.关键词
HIV/gp120/外泌体/巨噬细胞/极化Key words
HIV/gp120/Exosomes/Macrophages/Polarization分类
医药卫生引用本文复制引用
韦武均,庞晓霞,韦家住,陈晓昊,王春芳,黄晶晶,覃林秀,柴富,李嘉兴,林成,钟丽梅,黎作茶,胡仁统..经HIV包膜蛋白gp120刺激的T细胞外泌体促进巨噬细胞M2极化[J].海南医学院学报,2023,29(24):1841-1847,7.基金项目
National Natural Science Foundation Project(81960303) （81960303）
Baise City Scientific Research and Technology Development Plan Project(Encyclopedia 20213301,Encyclopedia 20213242,Encyclopedia 20194701) （Encyclopedia 20213301,Encyclopedia 20213242,Encyclopedia 20194701）
Guangxi Health Commission self funded research project(Z20190202,Z20211114) （Z20190202,Z20211114）
Guangxi University Young and Middle School Teachers'Basic Research Ability Enhancement Project(2021KY0538) （2021KY0538）
2022 Traditional Chinese Medicine Self funded Research Project(GXZYL20220304) （GXZYL20220304）
Open Project of Guangxi Key Laboratory of Molecular Pathology of Hepatobiliary Diseases(GXZDSYS-009) 国家自然科学基金项目(81960303) （GXZDSYS-009）
百色市科学研究与技术开发计划项目(百科20213301,百科20213242,百科20194701) （百科20213301,百科20213242,百科20194701）
广西卫生健康委员会自筹经费科研课题(Z20190202,Z20211114) （Z20190202,Z20211114）
广西高校中青年教师科研基础能力提升项目(2021KY0538) （2021KY0538）
2022年中医药自筹经费科研课题(GXZYL20220304) （GXZYL20220304）
广西肝胆疾病分子病理学重点实验室开放课题(GXZDSYS-009) （GXZDSYS-009）
