海南医学院学报2023，Vol.29Issue(24)：1848-1854,7.DOI:10.13210/j.cnki.jhmu.20231011.002
巨噬细胞外泌体介导mir-222靶向Caspase-10促进胶质瘤增殖
Macrophage-derived exosomes mediate mir-222 targeting Caspase-10 to promote glioma proliferation
摘要
Abstract
Objective:To investigate whether macrophage-derived exosomal mir-222 regulates Caspase-10 and whether macrophage-derived exosomes promote glioma proliferation by targeting Caspase-10 through mir-222.Methods:Macrophages were transfected with mir-222 mimics,and then macrophage-derived exosomes carrying mir-222 were transfected into glioma cells.Caspase-10 overexpression vector was constructed on this basis.RT-PCR was used to detect mir-222 expression in macro-phages and glioma cells.CCK-8 assay was used to detect the proliferative activity of glioma cells.Dual-luciferase reporter assay was used to verify the targeting binding of mir-222 with Caspase-10 gene.Western blot was used to detect Caspase-10 expression.Results:Transmission electron microscopy showed that macrophage-derived exosomes had spherical structures with a diameter of about 100 nm;mir-222 mimic significantly promoted the expression of mir-222 in macrophages;macrophage-derived exosomal mir-222 increased mir-222 expression and promoted proliferation in glioma cells;mir-222 could target bind with Caspase-10;mir-222 mimic significantly inhibited Caspase-10 expression.Conclusion:Macrohpage-derived exosomal mir-222 plays an important role in promoting glioma proliferation by downregulating Caspase-10 expression through targeted binding.关键词
胶质瘤/外泌体/mir-222/Caspase-10/巨噬细胞Key words
Glioma/Exosome/mir-222/Caspase-10/Macrophage分类
医药卫生引用本文复制引用
郑忠涛,祝叶,刘小强,王闯..巨噬细胞外泌体介导mir-222靶向Caspase-10促进胶质瘤增殖[J].海南医学院学报,2023,29(24):1848-1854,7.基金项目
This study was supported by Natural Science Foundation of Hainan(822MS205) （822MS205）
Hainan Provincial Health Industry Research Project (21A200288)海南省自然科学基金(822MS205) （21A200288）
海南省卫生健康行业科研项目(21A200288) （21A200288）
