海南医学院学报2023，Vol.29Issue(24)：1863-1869,7.DOI:10.13210/j.cnki.jhmu.20231023.002
基于BRD4/NF-κB/NLRP3通路介导的巨噬细胞焦亡探讨三石汤抑制痛风性关节炎的分子机制研究
Mechanism of Sanshi decoction inhibits macrophage pyroptosis by inhibiting BRD4/NF-κB/NLRP3 pathway in the treatment of gouty arthritis
摘要
Abstract
Objective:To observe the effect of Sanshi decoction on BRD4/NF-κB/NLRP3 pathway-mediated macrophage pyroptosis,so as to elucidate the molecular mechanism of Sanshi decoction in the treatment of gouty arthritis.Methods:THP-1 was induced into macrophages with foboside and the divided into the control group,model group,low-dose,medium-dose,high-dose group of Sanshi decoction,and BRD4 inhibitor group.Except for the control group,the remaining groups were induced with monosodium urate crystals to construct a gouty arthritis cell model.The activity of macrophages was detected by CCK8,the level of macrophage pyroptosis was detected by flow cytometry,the activity of LDH,the content of IL-1β and IL-18 were detected by enzyme-linked immunosorbent assay,and the expression of related proteins in the BRD4/NF-κB/NLRP3 pathway was detected by Western blot.Results:Compared with the control group,macrophage activity was decreased in the model group,and the level of pyroptosis,LDH activity,contents of IL-1β and IL-18,expression levels of BRD4,p-NF-κB p65,NLRP3,Caspase-1 p20,and IL-1β protein were significantly up-regulated,the differences were statistically significant(P<0.05).Compared with the mod-el group,macrophage activity was up-regulated in the Sanshi Decoction,and the level of pyroptosis,LDH activity,IL-1β and IL-18 contents,expression levels of BRD4,p-NF-κB p65,NLRP3,Caspase-1 p20,and IL-1β protein were significantly decreased with statistically significant differences(P<0.05).Conclusion:Sanshi decoction inhibits macrophage pyroptosis by inhibiting BRD4/NF-κB/NLRP3 pathway activation,thus improving the inflammation level of gouty arthritis.关键词
痛风性关节炎/巨噬细胞/细胞焦亡/BRD4/NF-κB/NLRP3通路/三石汤Key words
Gouty arthritis/Macrophage/Pyroptosis/BRD4/NF-κB/NLRP3 pathway/Sanshi decoction分类
医药卫生引用本文复制引用
朴勇洙,齐明明,聂双莲,潘国雄,张皓,王欣波..基于BRD4/NF-κB/NLRP3通路介导的巨噬细胞焦亡探讨三石汤抑制痛风性关节炎的分子机制研究[J].海南医学院学报,2023,29(24):1863-1869,7.基金项目
This study was supported by Heilongjiang Province Traditional Chinese Medicine Research Project(ZHY19-006) 黑龙江省中医药科研项目(ZHY19-006) （ZHY19-006）
