河北工业科技2023，Vol.40Issue(6)：436-444,9.DOI:10.7535/hbgykj.2023yx06005
两性霉素B菌种选育及发酵工艺研究
Study on breeding and fermentation technology of amphotericin B strain
随子华 1孙术超 2鞠加学 1葛鹍鹏 2赵国忠 1陈瑶瑶1
作者信息
- 1. 华北制药华胜有限公司,河北石家庄 052160
- 2. 微生物药物国家工程研究中心,河北石家庄 052165||河北省工业微生物代谢工程技术研究中心,河北石家庄 052165||华北制药集团新药研究开发有限责任公司,河北石家庄 052165
- 折叠
摘要
Abstract
In order to increase the yield of amphotericin B(AmB),reduce production costs and meet market demand,the combination of ultraviolet mutagenesis and atmospheric and room temperature plasma(ARTP)mutagenesis was used to select the strains.First,complex mutagenesis was performed on the starting strain A318-10 of Streptomyces nodosus.Then,the high-yield strains were screened and the optimal fermentation formula was obtained by Box-Behnken Design test on the significant effect factors using Design-Expert 12.Finally,fermentation optimization formula was verified in 1 L shaker and 50 L fermenter.The results show that the high-yielding strain 20-2-15 is obtained after ultraviolet mutagenesis for 60 s and ARTP mutagenesis for 35 s.The optimal fermentation formula is 94.6 g/L glucose,27.7 g/L medium temperature soybean powder and 24.1 g/L starch.Under the optimal formula,the maximum fermentation levels of 1 L shaker and 50 L fermenter are 18 968 μg/mL and 21 257 μg/mL,respectively,which are increased by 32.6%and 29.4%compared with that of the original formula.The results of the study are helpful to reduce the cost and improve the competitiveness of enterprises after being applied to large-scale production.关键词
发酵工程/两性霉素B/常压室温等离子体/紫外诱变/响应面筛选Key words
fermentation engineering/amphotericin B/atmospheric and room temperature plasma(ARTP)/ultraviolet induction/response surface screening分类
化学化工引用本文复制引用
随子华,孙术超,鞠加学,葛鹍鹏,赵国忠,陈瑶瑶..两性霉素B菌种选育及发酵工艺研究[J].河北工业科技,2023,40(6):436-444,9.
