河北医学2023，Vol.29Issue(12)：1941-1946,6.DOI:10.3969/j.issn.1006-6233.2023.12.02
Gas6/MerTK通路诱导线粒体自噬在肺癌进展的机制研究
Mechanistic of Gas6/MerTK Pathway Induces Mitochondrial Autophagy in the Progression of Lung Cancer
摘要
Abstract
Objective:To investigate the possible mechanism of mitochondrial autophagy in the progres-sion of lung cancer by downregulating the Gas6/MerTK signaling pathway.Methods:Calu-1 and A-427 cell lines were cultured and divided into(1)control group,Gas6 intervention(downregulation of Gas6)group,(2)control group,rapamycin(autophagy inducer,RAPA)group.Western blotting was used to detect the protein expression levels of Gas6,MerTK,LC3Ⅱ,and Beclin1.Transmission electron microscopy was em-ployed to observe autophagic vesicles in cells.Colony formation assay was conducted to assess the cloning abil-ity of Calu-1 and A-427 cells.CCK-8 assay was performed to measure cell viability.Scratch assay was used to evaluate the migration ability of Calu-1 and A-427 cells.Immunofluorescence was utilized to detect the ex-pression levels of LC3 in Calu-1 and A-427 cells.Results:Compared with the control group,the Gas6 inter-vention group showed a significant decrease in Gas6 and MerTK protein levels in Calu-1 and A-427 cells(P<0.05),a significant increase in LC3Ⅱ and Beclin1 protein levels(P<0.05),and the formation of autoph-agic vesicles with abundant autophagic vacuole accumulation.The cloning ability,cell viability,and migration distance of Calu-1 and A-427 cells were significantly reduced(P<0.05).Compared with the control group,the RAPA group exhibited an increase in LC3 protein expression in Calu-1 and A-427 cells(P<0.05).The cloning ability,cell viability,and migration distance of Calu-1 and A-427 cells were significantly reduced(P<0.05).Conclusion:Downregulating the Gas6/MerTK signaling pathway can induce mitochondrial auto-phagy in Calu-1 and A-427 cells and inhibit their proliferation,activity,and migration abilities.关键词
Gas6/MerTK信号通路/线粒体自噬/肺癌进展Key words
Gas6/MerTK signal path/Mitochondrial autophagy/Lung cancer progression引用本文复制引用
陆勤,张婷婷,陈明治..Gas6/MerTK通路诱导线粒体自噬在肺癌进展的机制研究[J].河北医学,2023,29(12):1941-1946,6.基金项目
江苏省卫生健康委员会科研项目,(编号:W20210189210) （编号:W20210189210）
