河北医学2023，Vol.29Issue(12)：1946-1951,6.DOI:10.3969/j.issn.1006-6233.2023.12.03
miR-190和ANXA11对直肠癌新辅助放化疗效用评估价值及相关性分析
Evaluation and Correlation Analysis of the Therapeutic Value of miR-190 and ANXA11 in Neoadjuvant Chemoradiotherapy for Rectal Cancer
摘要
Abstract
Objective:To analyze the roles and relationship of microRNA 190(miR-190)and mem-brane-associated protein A11(ANXA11)in the evaluation of neoadjuvant radiotherapy(NAC)for rectal cancer.Methods:A total of 148 patients who were diagnosed with rectal cancer and received treatment in our hospital from June 2019 through August 2022 were selected as the study subjects and included in the rectal cancer group,and 150 healthy people who underwent general physical examination in our hospital during the same period were selected as the control group,and real-time fluorescence quantitative detection(RT-PCR)detected the expression of serum miR-190 and ANXA11 mRNA in the two groups.All rectal cancer patients were treated with the same neoadjuvant radiotherapy and chemotherapy(NAC)before surgery,and were di-vided into the excellent efficacy group(n=89)and the poor efficacy group(n=59)according to the efficacy assessment criteria,and the serum miR-190 and ANXA11 mRNA expression of the patients in the two groups were compared.Spearman's correlation coefficient was used to analyze the relationship between serum miR-190,ANXA11 mRNA expression and clinical efficacy;Kaplan-meier was plotted to observe the effect of high and low miR-190,ANXA11 mRNA expression on the clinical efficacy of NAC;Subject operating characteris-tic curves(ROCs)were plotted to analyze the value of miR-190,ANXA11 single and combined assays for clinical utility assessment of NAC.Results:Compared with the control group,miR-190 expression was sig-nificantly lower,while ANXA11 mRNA expression was significantly higher in rectal cancer patients(0.87±0.18 vs 1.53±0.30,2.00±0.68 vs 0.95±0.09,t=-22.719,18.731,P<0.05).Compared with the poor-ef-ficacy group,miR-190 expression was significantly higher in patients in the excellent-efficacy group,while ANXA11 mRNA expression was significantly lower(0.96±0.15vs0.73±0.15,1.81±0.47vs2.28±0.83,t= 8.932,-3.958,all P<0.05);Spearman correlation coefficient analysis showed that miR-190 was positively correlated with NAC efficacy,while ANXA11 mRNA expression was negatively correlated with NAC efficacy(P<0.05);Kaplan-meier curves showed that the NAC efficacy excellence rate of the patients in the miR-190 high-expression group was significantly higher than that of the patients in the miR-190 low-expression group(χ2=39.120,P<0.001),and the excellent rate of NAC efficacy in patients in the ANXA11 mRNA low expression group was significantly higher than that in the ANXA11 mRNA high expression group(χ2=8.772,P=0.003);the ROC showed that the area under the curve(AUC)of the combined assay of miR-190 and ANXA11 was significantly higher than that of the single assay(P<0.05),and the sensitivity was 84.75%,the specificity of 87.64%.Conclusion:miR-190 and ANXA11 have specific expression levels in pa-tients with different NAC efficacy,and there is a close association between them and NAC efficacy.miR-190 and ANXA11 detection can effectively predict the clinical utility of NAC by combining miR-190 and ANXA11 assays,which can provide a new idea for the clinical assessment of rectal cancer efficacy.关键词
直肠癌/miR-190/膜联蛋白A11Key words
Rrectal cancer/miR-190/Membrane-bound protein A11引用本文复制引用
徐钰,胡锡池,赵于天..miR-190和ANXA11对直肠癌新辅助放化疗效用评估价值及相关性分析[J].河北医学,2023,29(12):1946-1951,6.基金项目
江苏省卫生健康委医学科研项目,(编号:2020215) （编号:2020215）
