河北医学2023，Vol.29Issue(12)：1951-1956,6.DOI:10.3969/j.issn.1006-6233.2023.12.04
血清外泌体LncRNA作为强直性脊柱炎早期诊断分子标记物的筛选研究
Screening Study on Serum Exosomal LncRNA as Early Diagnostic Molecular Markers for Ankylosing Spondylitis
摘要
Abstract
Objective:To screen serum exosomal long non-coding RNA(LncRNA)as potential molec-ular markers for the early diagnosis of ankylosing spondylitis(AS).Methods:Whole blood samples of AS pa-tients in our hospital from January 2021 to December 2022 were collected.Exosomes were isolated from the plasma of AS patients(n=50)and healthy individuals(n=50)by ultrafast centrifuge.Total exosomal RNA was extracted using TRIzol reagent.LncRNAs were analyzed by microarray analysis in the two groups of pa-tients,and the expression levels of LncRNAs in all patients and healthy subjects were measured by real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Results:The results of this study showed that six lncrnas(LNCV6_116109、LNCV6_98390、LNCV6_38772、LNCV6_108266、LNCV6_84003 和LNCV6_98602)were significantly up-regulated in AS patients by qRT-PCR.Not a healthy individual.Re-ceiver operating characteristic curve(ROC)was used to verify its diagnostic accuracy.The area values under the curve of lncRNA were 0.8052(LNCV6_116109)、0.7088(LNCV6_98390)、0.7460(LNCV6_38772)、0.7292(LNCV6_108266)、0.7356(LNCV6_84003)and 0.6800(LNCV6_98602.Conclusion:This study showed that the expression of exosome LncRNAs(LNCV6_84003,LNCV6_98602,LNCV6_108266,LNCV6_116109,LNCV6_98390 and LNCV6_38772)was significantly up-regulated in plasma of patients with AS.They may serve AS potential non-invasive biomarkers for early diagnosis of AS.关键词
外泌体/LncRNA/强直性脊柱炎Key words
Exosome/LncRNA/Ankylosing spondylitis引用本文复制引用
华凤伟,于阔,王家琦..血清外泌体LncRNA作为强直性脊柱炎早期诊断分子标记物的筛选研究[J].河北医学,2023,29(12):1951-1956,6.基金项目
黑龙江省卫生健康委员会科研项目,(编号:H202250104) （编号:H202250104）
