河北医学2023，Vol.29Issue(12)：1957-1962,6.DOI:10.3969/j.issn.1006-6233.2023.12.05
血清外周miRNAs差异表达在脊柱结核中的临床意义
Clinical Significance of Differential Expression of Serum Peripheral miRNAs in Spinal Tuberculosis
摘要
Abstract
Objective:To explore the clinical significance of differential expression of serum peripheral micrornas(miRNAs)in spinal tuberculosis.Methods:From May 2020 to May 2023,50 patients with spinal tuberculosis(experimental group)and 30 healthy people(control group)admitted to the Department of Spinal Orthopaedics of our hospital were taken as the research objects.MiRCURYTM LNA array(v.18.0)was used to screen the expression profile of miRNAs in 10 experimental patients and 10 healthy controls.The expression levels of 10 selected miRNAs were verified by RT-qCPR in the research cohort.Hsa-miR-4708-3p,hsa-miR-5100 and hsa-miR-493-3p were selected to construct the spinal tuberculosis diagnosis model(STB model)based on miRNAs.Diagnostic value of STB model in spinal tuberculosis by analyzing receiver operat-ing characteristic(ROC)curve.Results:Compared with healthy people,288 kinds of miRNAs were found in the serum of patients with spinal tuberculosis(123 kinds were up-regulated and 165 kinds were down-regula-ted)(multiple change>2,P<0.05).RT-qPCR analysis confirmed that compared with the control group,the levels of hsa-miR-4708-3p and hsa-miR-5100 in serum samples of the experimental group increased signifi-cantly(P<0.05),and the level of hsa-miR-493-3p decreased significantly(P<0.05).Based on the expres-sion levels of hsa-miR-4708-3p,hsa-miR-5100 and hsa-miR-493-3p as variables,a multivariate logistic regression model was established as follows:0.582+1.055×(hsa-miR-4708-3p)+0.811×(hsa-miR-5100)-0.970×(hsa-miR-493-3p).Compared with the control group,the STB model score in the experi-mental group was significantly higher(0.32±0.04 vs 0.65±0.08,t=6.09,P<0.001).When the critical value was set to 0.46,the area under ROC curve(AUC)of STB model in identifying spinal tuberculosis was 0.911,with sensitivity of 88.9%and specificity of 77.0%.The STB model scores of patients with spinal tu-berculosis with abnormal CRP and erythrocyte sedimentation rate were significantly higher than those with nor-mal CRP and erythrocyte sedimentation rate(P<0.05).The score of STB model was positively correlated with CRP and erythrocyte sedimentation rate(r=0.633,0.499,all P<0.001).Conclusion:Hsa-miR-4708-3p,hsa-miR-5100 and hsa-miR-493-3p have stable differential expressions in peripheral blood of patients with spinal tuberculosis,and the diagnostic model containing these three miRNAs has good diagnostic perform-ance for spinal tuberculosis.关键词
脊柱结核/外周血/微小RNA/诊断模型/差异表达Key words
Spinal tuberculosis/Peripheral blood/MicroRNA/Diagnostic model/Difference show引用本文复制引用
付海平,王飞,郇颜强,阿拉木苏..血清外周miRNAs差异表达在脊柱结核中的临床意义[J].河北医学,2023,29(12):1957-1962,6.基金项目
2021年度内蒙古自治区卫生健康科技计划项目,(编号:202101013) （编号:202101013）
