河北医学2023，Vol.29Issue(12)：2004-2011,8.DOI:10.3969/j.issn.1006-6233.2023.12.013
LncRNA PINK1-AS调节miR-134-5p/SERPINE1轴对胃癌细胞增殖凋亡和上皮间质转化的影响
Effects of LncRNA PINK1-AS on the Proliferation Apoptosis and Epithelial Mesenchymal Transformation of Gastric Cancer Cells by Regulating the miR-134-5p/SERPINE1 Axis
摘要
Abstract
Objective:To investigate the effects of LncRNA PINK1-AS on the proliferation,apoptosis and epithelial mesenchymal transformation(EMT)of gastric cancer cells by regulating the miR-134-5p/ser-ine protease inhibitor clade E member 1(SERPINE1)axis.Methods:QRT-PCR was applied to detect the expression levels of PINK1-AS,miR-134-5p,and SERPINE mRNA in 60 cases of gastric cancer tissues and 60 cases of adjacent tissues.Gastric cancer cells MKN28 were cultured in vitro and grouped into control group,si-NC group,si-PINK1-AS group,pc-NC group,pc-PINK1-AS group,miR-NC group,and miR-134-5p mimics group;QRT-PCR was applied to detect the expression levels of PINK1-AS,miR-134-5p,and SERPINE mRNA in cells of each transfection group,Cck-8 was applied to detect cell proliferation;Flow cytometry was applied to detect cell apoptosis rate;Transwell test was applied to detect cell migration and in-vasion;Western blot was applied to detect the expression levels of SERPINE1,Ki-67,cleaved caspase-3,E-cadherin,N-cadherin,and vimentin proteins in cells.Double Luciferase reporter gene experiment was ap-plied to detect the targeting relationship between PINK1-AS and miR-134-5p,and between miR-134-5p and LncRNA SERPINE1.Results:The expression of PINK1-AS and SERPINE mRNA in gastric cancer tis-sue was obviously higher than that in adjacent tissues,while the expression of miR-134-5p was lower than that in adjacent tissues(P<0.05).Down-regulation the expression of PINK1-AS can reduce the PINK1-AS,SERPINE mRNA,OD450 values(24,48h),cell migration and invasion,the expression of SERPINE1,Ki-67,N-cadherin,vimentin proteins in MKN28 cells,increased the expression of miR-134-5p,cell apop-tosis rate,the expression of cleaved caspase-3,and E-cadherin proteins(P<0.05).Overexpression of PINK1-AS can increase the PINK1-AS,SERPINE mRNA,OD450 values(24,48h),cell migration and in-vasion,the expression of SERPINE1,Ki-67,N-cadherin,vimentin proteins in MKN28 cells,the expression of miR-134-5p,cell apoptosis rate,the expression of cleaved caspase-3,and E-cadherin proteins obviously decreased(P<0.05).Overexpression of miR-134-5p increased miR-134-5p expression,apoptosis rate,cleaved caspase-3,and E-cadherin protein expression,and decreased SERPINE mRNA,OD450 value(24,48h),cell migration and invasion number,SERPINE1,Ki-67,N-cadherin,and vimentin protein expres-sion(P<0.05).Conclusion:LncRNA PINK1-AS can regulate the miR-134-5p/SERPINE1 axis,thereby affecting proliferation,apoptosis and EMT of gastric cancer cells.关键词
胃癌细胞/LncRNA PINK1-AS/miR-134-5p/SERPINE1轴/上皮间质转化Key words
Gastric cancer cells/LncRNA PINK1-AS/miR-134-5p/SERPINE1 axis/Epi-thelial mesenchymal transformation引用本文复制引用
李凡,宋玉涛,王峰,冯浩..LncRNA PINK1-AS调节miR-134-5p/SERPINE1轴对胃癌细胞增殖凋亡和上皮间质转化的影响[J].河北医学,2023,29(12):2004-2011,8.基金项目
河北省医学科学研究课题,(编号:20220437) （编号:20220437）
