河南农业大学学报2023，Vol.57Issue(6)：936-948,13.DOI:10.16445/j.cnki.1000-2340.20231020.001
桑树Aux/IAA基因家族鉴定与IBA处理下表达模式分析
Identification of Aux/IAA gene family of Morus notabilis and analysis of expression patterns under IBA treatment
摘要
Abstract
[Objective]To identify Aux/IAA gene family members in the whole genome,and study the effects of IBA treatment on the gene expression pattern of the family,so as to provide a basis for further research on the function of Aux/IAA gene family in Morus notabilis.[Method]The Aux/IAA gene family members of Morus notabilis were identified and analyzed through local Morus notabilis whole genome data,including protein physicochemical property analysis,phylogenetic tree construction,gene structure analysis,protein tertiary structure and protein interaction association analysis,and promoter cis-acting element analysis.The cuttings of"Qiangsang No.1"were treated with 1 000 mg·L-1 IBA and water control,and the expression patterns of MnAux/IAA gene family on the 10th,20th,30th and 40th day repectively after treatment were analyzed.[Result]The MnAux/IAA gene family has 51 members,which are divided into 8 subfamilies,and the AtIAA gene family is distributed in 6 subfamilies;All 51 gene family members had exons and introns,and only 1 member had inexistence UTR;The cis-acting elements indicated that the MnAux/IAA gene family had corresponding regulatory effects on light,hormones and stress;The similarity of gene structure of protein isogenetic sequences is high,but there exists greater difference between families;After IBA treatment,the expression levels of most genes were increased to varying degrees,and the expression levels of genes(MnAux/IAA34,MnAux/IAA33,MnAux/IAA32)were decreased to a large extent.[Conclusion]The function of the MnAux/IAA gene family is relatively stable,and there is no significant change in the family evolution;IBA can promote the expression of Aux/IAA gene;MnAux/IAA34,MnAux/IAA33,MnAux/IAA32 have high homology with AtARF2,which suggests that they may play a role in regulating leaf senescence.关键词
桑树/Aux/IAA基因家族/进化分析/基因表达/生物信息学Key words
Morus notabilis/Aux/IAA gene family/evolutionary analysis/gene expression/bioinfor-matics分类
农业科技引用本文复制引用
冯曦,豆浩,孙佳佳,孙慧娟,毕会涛..桑树Aux/IAA基因家族鉴定与IBA处理下表达模式分析[J].河南农业大学学报,2023,57(6):936-948,13.基金项目
河南省桑树种质资源建设项目(30802649) （30802649）
河南省林木种质资源调查专项项目(30601537) （30601537）
