河南农业大学学报2023，Vol.57Issue(6)：958-966,9.DOI:10.16445/j.cnki.1000-2340.20231019.001
桃PpILR1基因调控分枝角度的功能解析
Function analysis of PpILR1 gene regulating branch angle in peach
摘要
Abstract
[Object]In order to provide theoretical basis for genetic improvement of peach tree architecture,the function of PpILR1 was verified,and the molecular mechanism of auxin response factor PpARF4 and PpARF8A to regulate branching angle by binding and inhibiting PpILR1 promoter activity was explored.[Method]The overexpression vector of PpILR1 was constructed,and was used for stable transformation in micro-Tom tomato.The promoter sequences of PpILR1 gene and the CDS sequence of PpARF4 and PpARF8A genes were cloned,and the regulation relationship between them was further verified via yeast single hybridization test and tobacco transient expression test.[Result]Compared with the wild type tomato,the two transgenic lines of the overexpression PpILR1 significantly reduced branch angle.Y1H results suggested that PpARF4 and PpARF8A transcription factors could bind to the promoter of PpILR1,and the transient expression assay in tobacco leaves indicated that PpARF4 and PpARF8A were transcriptional suppressor,which could obviously inhibit the activity of PpILR1.[Conclusion]The overexpression of PpILR1 gene resulted in the significant reduction of branch angle,which indicated that PpILR1 may participate in regulating the branch angle.关键词
桃/分枝角度/生长素响应因子/生长素酰胺水解酶Key words
peach/branch angle/auxin response factor/IAA-leucine resistant1-like hydrolase分类
农业科技引用本文复制引用
张杰,冯建灿,沈芮先,裴弯,谢贺芳,王小贝,郑先波,连晓东,张海朋,谭彬..桃PpILR1基因调控分枝角度的功能解析[J].河南农业大学学报,2023,57(6):958-966,9.基金项目
国家自然科学基金青年基金项目(32202412) （32202412）
河南省重大公益科技专项项目(201300110500) （201300110500）
河南农业大学科技创新基金项目(KJCX2020A15) （KJCX2020A15）
河南省现代农业产业技术体系建设项目(HARS-22-09-G1) （HARS-22-09-G1）
