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混合池测序在水稻深根比QTL验证中的应用研究

鲍泽然

黑龙江农业科学Issue(12):1-6,6.
黑龙江农业科学Issue(12):1-6,6.DOI:10.11942/j.issn1002-2767.2023.12.0001

混合池测序在水稻深根比QTL验证中的应用研究

Application of Mixed Pool Sequencing in QTL Verification of Rice Deep Root Ratio

鲍泽然1

作者信息

  • 1. 黑龙江省农垦科学院水稻研究所,黑龙江佳木斯 154000
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摘要

Abstract

The root system of rice is a key organ for nutrient absorption and growth,and a strong root system is an important indicator for cultivating drought resistance in rice.In order to cultivate excellent rice varieties,mixed pool sequencing method was used to sequence rice samples,and sample screening and labeling were carriedout through inter parent polymorphism to mine quantitative trait loci(QTLs)for root traits in rice.The results showed that among the parental polymorphism markers,486 SSR molecular markers were used to analyze parental polymorphism,with the highest proportion of polymorphism being 43.20%.In QTL mapping of rice,5 candidate genes were indentified to be specifically expressed at the root tip and related to the growth angle of the root tip,indicating a close relationship between these genes and the deep root ratio of rice.

关键词

水稻/QTL/混合池测序/深根比/基因

Key words

rice/QTL/mixing cell sequencing/depth root ratio/gene

引用本文复制引用

鲍泽然..混合池测序在水稻深根比QTL验证中的应用研究[J].黑龙江农业科学,2023,(12):1-6,6.

基金项目

国家水稻产业技术体系(CARS-01-58). (CARS-01-58)

黑龙江农业科学

1002-2767

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