河南农业科学2023，Vol.52Issue(12)：42-48,7.DOI:10.15933/j.cnki.1004-3268.2023.12.005
不同IAA和6-BA质量浓度组合对甘薯茎尖培养和表型变异的影响
Effect of Combinations with Different Concentrations of IAA and 6-BA on Shoot Tip Culture and Phenotypic Variation of Sweetpotato
摘要
Abstract
The shoot tips of sweetpotato variety Yanshu 29 were used as explants and MS medium as basic medium to study the callus induction,buds differentiation,plants regeneration and phenotypic variation under different concentrations of indole-3-acetic acid(IAA)(0.1 and 0.5 mg/L)and 6-benzylaminopurine(6-BA)(0.1,0.5 and 1.0 mg/L).The results showed that when the IAA concentration in the basic MS medium was 0.5 mg/L,the callus induction effect was the best,shoot tip survival rate was the highest,and the average callus induction rate and shoot tip survival rate of Yanshu 29 were 92.00% and 88.00%,respectively.The bud induction rate and tufted buds induction rate generally increased with the increase of 6-BA concentration in the medium.Under 0.1 mg/L IAA,the average induction rate of buds and tufted buds reached 64.27% and 80.56%,respectively.Addition of 0.5 mg/L IAA in medium was beneficial to plants regeneration,with the average plant regeneration rate of 39.33%;under this IAA concentration,the plant regeneration rate and number of regeneration plant increased,and plant regeneration cycle decreased with the increase of 6-BA concentration.A somaclonal variation line,named YM6-11,was found from regeneration plants,and its leaf vein color,flower shape and cross-compatibility were different compared with Yanshu 29.In conclusion,the most suitable medium for shoot tip culture of Yanshu 29 is MS+0.5 mg/L IAA+1.0 mg/L 6-BA,and the phenotypic and genetic identification should be performed when the regenerated plants are used for actual production,so as to reduce unnecessary economic losses.关键词
甘薯/茎尖培养/愈伤诱导/植株再生/无性系变异/IAA/6-BAKey words
Sweetpotato/Shoot tip culture/Callus induction/Plant regeneration/Somaclonal variation/IAA/6-BA分类
农业科技引用本文复制引用
贾礼聪,王连军,杨子桐,商丽丽,张磊,邱鹏飞,王翠娟,韩俊杰,辛国胜..不同IAA和6-BA质量浓度组合对甘薯茎尖培养和表型变异的影响[J].河南农业科学,2023,52(12):42-48,7.基金项目
粮食作物种质创新与遗传改良湖北省重点实验室开放课题项目(2020lzjj06) （2020lzjj06）
国家甘薯产业技术体系项目(CARS-10) （CARS-10）
湖北省支持种业高质量发展资金项目(HBZY2023B002) （HBZY2023B002）
