河南农业科学2023，Vol.52Issue(12)：136-141,6.DOI:10.15933/j.cnki.1004-3268.2023.12.015
减毒鼠伤寒沙门菌Ⅲ型分泌系统介导Apoptin对肿瘤细胞糖酵解的影响
Effect of Apoptin Mediated by Attenuated Salmonella typhimurium Type Ⅲ Secretion System on Glycolysis in Tumor Cells
摘要
Abstract
To further investigate the effect of Apoptin mediated by recombinant attenuated Salmonella typhimurium type Ⅲ secretion system on glycolysis of tumor cells,B16F10 cells were infected with recombinant strain∆crp∆asd SL1344(pYA3493-SopE-Apoptin)or complementary strain∆crp∆asd SL1344(pYA3493-SopE),and the cells were collected at 12 h and 24 h after infection to detect the levels of pyruvic acid,lactic acid,pyruvate kinase and hexokinase.The results showed that the intracellular pyruvate level of recombinant strain group was significantly reduced(P<0.01).At 24 h after infection,the lactic acid content of recombinant strain group was significantly lower than that of complementary strain group and cell control group(P<0.01).In addition,the pyruvate kinase activity and hexokinase activity of recombinant strain group were significantly lower than that of complementary strain group and cell control group(P<0.05).These results suggest that recombinant strain can inhibit the glycolysis ability of B16F10 cells by down-regulating the pyruvate and lactic acid content and reducing the activities of pyruvate kinase and hexokinase,leading to significant metabolic changes in tumor cells.关键词
凋亡素/糖酵解/减毒鼠伤寒沙门菌/凋亡/肿瘤细胞Key words
Apoptin/Glycolysis/Attenuated Salmonella typhimurium/Apoptosis/Tumor cells分类
农业科技引用本文复制引用
郁川,石胜丽,朱文文,胡立中,宋敏杰,李静雅,张春杰..减毒鼠伤寒沙门菌Ⅲ型分泌系统介导Apoptin对肿瘤细胞糖酵解的影响[J].河南农业科学,2023,52(12):136-141,6.基金项目
国家自然科学基金项目(31302059) （31302059）
河南省高等学校重点科研项目(22A230015) （22A230015）
