湖南中医药大学学报2023，Vol.43Issue(12)：2150-2156,7.DOI:10.3969/j.issn.1674-070X.2023.12.002
壮骨止痛胶囊对去卵巢致骨质疏松大鼠肠道微生态的影响
Effects of Zhuanggu Zhitong Capsule on intestinal microecology of ovariectomised rats with osteoporosis
摘要
Abstract
Objective To investigate the effects of Zhuanggu Zhitong Capsule(ZGZTC)on the intestinal flora of rats with ovariectomized(OVX)-induced osteoporosis(OP).Methods Female SD rats were selected and randomized into ten in the sham-operated(SHAM)group and 30 in the modeling group.In the modeling group,both ovaries were excised,and in the SHAM group,only an equal mass of adipose tissue around the ovaries was excised.One week later,the modeling group was randomized into the OVX group,the estradiol valerate(EV)group,and the ZGZTC group,with ten rats in each.The EV group was administered EV suspension 0.09 mg/(kg·d),the ZGZTC group was administered ZGZTC suspension 1.944 g/(kg·d),and the SHAM group and OVX group were administered equal volume of saline.The material was taken after 12 weeks of drug administration.The structural changes of femur and colon were observed by HE staining,the expression of zonula occludens-1(ZO-1)and Occludin mRNA in colon was analyzed by RT-PCR,the expression of serum interleukin-17(IL-17),transforming growth factor-β(TGF-β)and lipopolysaccharide(LPS)was determined by ELISA.The feces of rats were collected for 16S rRNA sequencing of intestinal flora,to analyze the differences of intestinal microbiota.Results Compared with the SHAM group,in the OVX group the femoral trabeculae of rats were sparse and broken,and the morphology of colonic epithelial cells was disrupted and infiltrated by inflammatory cells;compared with the OVX group,in the ZGZTC and EV groups the number of trabeculae increased and the infiltration of inflammatory cells in the colon was reduced.Compared with the SHAM group,in the OVX group the relative expression of Occludin and ZO-1 mRNA in the colon tissues of rats was significantly lower(P<0.01),the values of serum IL-17 and LPS were higher(P<0.01),and the value of TGF-β was lower(P<0.01).Compared with the OVX group,in the ZGZTC and EV groups the colon tissues of rats showed a significant increase in the expression of Occludin and ZO-1 mRNA relative expression(P<0.01),serum IL-17 and LPS values were reduced(P<0.01),and TGF-β values increased(P<0.01).Compared with the OVX group,in the ZGZTC and EV groups the abundance of thick-walled bacillus phylum was increased,and the abundance of anaplastic bacillus phylum was decreased.Conclusion ZGZTC can improve the bone microstructure of OVX rats,and its mechanism may be related to increasing the diversity of intestinal flora and regulating the structure of intestinal flora.关键词
去卵巢大鼠/壮骨止痛胶囊/绝经后骨质疏松/骨代谢/肠道微生态/16S rRNA/代谢产物Key words
ovariectomised rats/Zhuanggu Zhitong Capsule/postmenopausal osteoporosis/bone metabolism/intestinal flo-ra/16S rRNA/metabolite分类
医药卫生引用本文复制引用
张天慧,刘婷,雷晓明,王舒,王丰林,刘平安,张国民..壮骨止痛胶囊对去卵巢致骨质疏松大鼠肠道微生态的影响[J].湖南中医药大学学报,2023,43(12):2150-2156,7.基金项目
国家自然科学基金面上项目(81973920) （81973920）
湖南省中医药科研计划重点项目(E2022007) （E2022007）
湖南省教育厅科学研究重点项目(22A0260) （22A0260）
湖南中医药大学研究生科研创新项目(2022CX62) （2022CX62）
湖南省研究生科研创新项目(CX20220821) （CX20220821）
长沙市 2022年度指导性科技计划项目(kzd22005). （kzd22005）
