湖南中医药大学学报2023，Vol.43Issue(12)：2157-2162,6.DOI:10.3969/j.issn.1674-070X.2023.12.003
补肝健膝方对膝骨关节炎模型兔COMP、CTX-Ⅱ及炎性指标的影响
Effects of Bugan Jianxi Formula on COMP,CTX-Ⅱ,and inflammatory indexes in knee osteoarthritis model rabbits
摘要
Abstract
Objective To study the effects of Bugan Jianxi Formula(BGJXF)on levels of serum,interleukin-1(IL-1),and tumor necrosis factor-α level(TNF-α),and the content of cartilage oligomeric matrix protein(COMP)and C-telopeptide of type Ⅱ collagen(CTX-Ⅱ)in the serum,and to reveal the mechanism of action of BGJXF on knee osteoarthritis(KOA).Methods Rabbit KOA model was constructed through papain joint injection,and they were randomly divided into blank control group,model control group,Duhuo Jisheng Decoction(DHJSD)group,and low-,medium-,and high-dose BGJXF groups,with five rabbits in each group.After four weeks of modeling,the blank control and the model control groups received normal saline one time per day;DHJSD group received gastric administration of DHJSD 2.895 g/(kg·d)one time per day;low-,medium-,and high-dose BGJXF groups received gastric administration of BGJXF decoction[1.962,3.924,7.848 g/(kg·d)]once per day.After four weeks,the pathological changes in the cartilage of the knee were observed by HE staining;cartilage damage was assessed by Pelletier score;IL-1 and TNF-α levels of the serum and the articular cavity effusion,and COMP and CTX-Ⅱ content of the serum was measured by ELISA.Results Compared with the blank control group,the cartilage articular surface of the model control group was not smooth,mucoid changes could be seen locally in the cartilage,and the number of cartilage and deep cells was significantly reduced;the Pelletier score was significantly elevated(P<0.05);the content of IL-1 and TNF-α in the serum and articular cavity effusion as well as the content of COMP and CTX-Ⅱ in the serum were all elevated(P<0.05).Compared with the model control group,the cartilage surface levels were more clearly distributed and the tideline structure was intact in the DHJSD group and each dose group of BGJXF;the Pelletier score was reduced(P<0.05);the levels of IL-1 and TNF-α in serum and articular cavity effusion as well as the content of COMP and CTX-Ⅱin serum were all reduced(P<0.05).Compared with the DHJSD group,the cartilage surface level distribution of each dose group of BGJXF was clearer,and the tideline structure was more complete;the Pelletier score increased(P<0.05);the content of IL-1 and TNF-α in serum and articular cavity fluid,and the content of COMP and CTX-Ⅱ in serum decreased(P<0.05).Conclusion BGJXF can effectively improve the bone microstructure and regulate the synovial cell activity of knee articular in rabbits with KOA,which may be related to the inhibition of IL-1 and TNF-α levels in the serum and articular cavity fluid as well as the content of COMP and CTX-Ⅱ in serum.关键词
补肝健膝方/独活寄生汤/膝骨关节炎/软骨寡聚基质蛋白/Ⅱ型胶原C端肽/炎性指标Key words
Bugan Jianxi Formula/Duhuo Jisheng Decoction/knee osteoarthritis/cartilage oligo matrix protein/C-telopeptide of type Ⅱ collagen/inflammatory indexes分类
医药卫生引用本文复制引用
叶子丰,戎宽,匡建军,仇湘中,许辉..补肝健膝方对膝骨关节炎模型兔COMP、CTX-Ⅱ及炎性指标的影响[J].湖南中医药大学学报,2023,43(12):2157-2162,6.基金项目
国家中医药管理局"全国名老中医药专家仇湘中传承工作室建设项目"(国中医药人教[2016]42号) （国中医药人教[2016]42号）
湖南省科学技术厅临床创新引导项目(2020SK51002) （2020SK51002）
长沙市科技局项目(kq2004046) （kq2004046）
湖南省自然科学基金面上项目(2022JJ30359) （2022JJ30359）
湖南省中医药管理局重点课题项目(C2022018). （C2022018）
