湖南中医药大学学报2023，Vol.43Issue(12)：2177-2184,8.DOI:10.3969/j.issn.1674-070X.2023.12.006
益肾通癃颗粒调控Wnt/β-catenin信号通路对人前列腺癌PC3细胞荷瘤裸鼠的干预作用
Interventional effects of Yishen Tonglong Granules on regulating the Wnt/β-catenin signaling pathway in nude mice bearing human prostate cancer cell PC3 tumor
摘要
Abstract
Objective To observe the intervention effects of Yishen Tonglong Granules(YSTLG)on the Wnt/β-catenin signaling pathway in nude mice bearing human prostate cancer cell PC3 tumor.Methods The models of nude mice bearing prostate cancer tumor were prepared using human prostate cancer bone metastasis cell PC3 and were randomized into model control,positive drug,and low-,medium-,and high-dose YSTLG,and combination drug groups.Corresponding drug interventions were given for four consecutive weeks.After administration,the animals were sacrificed to obtain subcutaneous transplanted tumor tissue samples.HE staining was performed to observe pathological changes,immunohistochemistry was used to check cell proliferation,TUNEL was used to check cell apoptosis,and Western blot and immunohistochemistry were used to check the expression levels of Wnt signaling pathway-related gene proteins.Results YSTLG can effectively relieve the pathological changes of subcutaneous transplanted tumor tissue in nude mice bearing tumor,significantly inhibit the proliferation of tumor cells,and promote cell apoptosis with concentration-dependent effects.YSTLG can also downregulate the APC protein expressions of Wnt/β-catenin signaling pathway related genes including Wnt1,Wnt3a,and β-catenin(P<0.01),upregulate the GSK-3β protein expression of Wnt/β-catenin signaling pathway related gene(P<0.01),and reduce the activity of p-GSK-3β protein(P<0.01).The correlation between drug efficacy and dosage was positive,and the best effects are achieved when YSTLG was combined with the Wnt signaling pathway blocker ICG(P<0.01).Conclusion YSTLG could inhibit the proliferation of prostate cancer cells,promote their apoptosis,which are likely to be achieved by blocking Wnt/β-catenin signaling pathway.关键词
PC3细胞/前列腺癌/Wnt/β-catenin/益肾通癃颗粒/裸鼠Key words
Wnt/β-catenin/Yishen Tonglong Granules/PC3 cell/prostate cancer/nude mice分类
医药卫生引用本文复制引用
朱文雄,袁轶峰,彭涛,陈立蔓,陈其华,张熙..益肾通癃颗粒调控Wnt/β-catenin信号通路对人前列腺癌PC3细胞荷瘤裸鼠的干预作用[J].湖南中医药大学学报,2023,43(12):2177-2184,8.基金项目
湖南省中医药科研计划项目(2021108) （2021108）
湖南省卫生健康委员会科研课题(202103051239) （202103051239）
湖南省自然科学基金面上项目(2021JJ30396) （2021JJ30396）
湖南省自然科学基金青年基金项目(2022JJ40335). （2022JJ40335）
