湖南中医药大学学报2023，Vol.43Issue(12)：2191-2198,8.DOI:10.3969/j.issn.1674-070X.2023.12.008
岩黄连总生物碱对四氯化碳诱导肝纤维化大鼠的改善作用研究
Improvement effects of total alkaloids from Yanhuanglian(Corydalis saxicola Bunting)on carbon tetrachloride-induced liver fibrosis in rats
摘要
Abstract
Objective To investigate the protective effects and mechanism of total alkaloids of Yanhuanglian(Corydalis saxicola Bunting)(TACS)on carbon tetrachloride-induced liver fibrosis in rats.Methods The SD rats were injected intraperitoneally with 30%carbon tetrachloride(CCl4)olive oil solution(0.1 mL/100 g)to establish liver fibrosis model,twice a week for 12 consecutive weeks.Liver fibrosis rats were randomly divided into five groups with eight animals per group:model group,low-,medium-,and high-dose TACS(25,50,100 mg/kg)groups,and bifendate group(30 mg/kg).Additionally,a normal group(8 rats)was also set up.The normal group and model group were given constant volume of saline intragastrically,while the other groups were intervened by intragastric administration of the corresponding drug(1.0 mL/100 g)according to the set dose at the 11th week of modeling,once a day for two weeks.After 24 h of the last administration,the serum,liver and spleen tissues were collected to determine the liver and spleen indexes of rats.The pathological changes of liver tissues were observed via hematoxylin-eosin(HE)staining and Masson staining.Colorimetric method was used to check the content of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total protein(TP),albumin(ALB),malondialdehyde(MDA),superoxide dismutase(SOD),and Glutathione peroxidase(GSH-Px)in serum.The expression levels of serum hyaluronic acid(HA),laminin(LN),type Ⅲ procollagen(PCⅢ),collagen Ⅳ(Ⅳ-C),transforming growth factor-β1(TGF-β1),interleukin-1β(IL-1β),interleukin-8(IL-8),and interleukin-6(IL-6)in serum were measured by ELISA.Results Compared with the normal group,the rats in the model group were unresponsive,the liver and spleen indexes increased(P<0.01),the liver tissues showed obvious fibrosis,the levels of ALT,AST,MDA,HA,LN,PCⅢ,and Ⅳ-C increased significantly(P<0.01),the levels of TP,ALB,SOD,and GSH-Px in serum decreased significantly(P<0.01),and the expression levels of TGF-β1,IL-1β,IL-8,and IL-6 in serum were significantly up-regulated(P<0.01).Compared with the model group,the state and liver and spleen indexes of rats in medium-and high-dose TACS groups significantly improved(P<0.05,P<0.01),the pathological damage and fibrous hyperplasia of liver tissues were effectively regulated,the content of ALT,AST,and MDA in serum significantly decreased(P<0.01),the expression levels of TP,ALB,SOD,and GSH-Px significantly increased(P<0.05,P<0.01)and the expression levels of LN,HA,PCⅢ,Ⅳ-C,TGF-β1,IL-1β,IL-8,and IL-6 proteins significantly down-regulated(P<0.05,P<0.01).Conclusion TACS has obvious anti-hepatic fibrosis effects,and its mechanism of action may be related to antioxidation and inhibition of inflammatory factor expression.关键词
岩黄连/总生物碱/四氯化碳/肝纤维化/抗氧化/炎症因子Key words
Corydalis saxicola Bunting/total alkaloid/carbon tetrachloride/carbon tetrachloride liver fibrosis/antioxidation/inflammatory factor分类
医药卫生引用本文复制引用
覃妮,黄曙院,周柳,黄华萍,沈新辉,周琳,孙东琪,陆世银..岩黄连总生物碱对四氯化碳诱导肝纤维化大鼠的改善作用研究[J].湖南中医药大学学报,2023,43(12):2191-2198,8.基金项目
柳州市科技攻关与新产品试制(2020NBAA0802) （2020NBAA0802）
广西中医药大学自然科学基金面上项目(2020MS055). （2020MS055）
