湖南中医药大学学报2023，Vol.43Issue(12)：2199-2205,7.DOI:10.3969/j.issn.1674-070X.2023.12.009
基于HPLC和LC-Orbitrap-MS的栀子化学成分定性与定量研究
Qualitative and quantitative study on chemical constituents of Zhizi(Gardeniae Fructus)based on HPLC and LC-Orbitrap-MS
摘要
Abstract
Objective To qualitatively and quantitatively study the chemical constituents of Zhizi(Gardeniae Fructus),so as to provide references for its quality control and evaluation.Methods The main components of Zhizi(Gardeniae Fructus)were qualitatively and quantitatively analyzed by HPLC dual-wavelength method,and the chemical constituents in Zhizi(Gardeniae Fructus)were identified by LC-Orbitrap-MS combined with mzCloud and mzVault databases.Results The HPLC characteristic chromatogram of Zhizi(Gardeniae Fructus)was established,and 11 main components were identified by comparing with reference solution.Six of them were quantified,and 31 components were identified by LC-Orbitrap-MS.Conclusion The established HPLC quantitative method is stable and reliable,and can be used for the quality control of Zhizi(Gardeniae Fructus).Moreover,LC-Orbitrap-MS technology can achieve rapid full-component analysis of Zhizi(Gardeniae Fructus),providing a scientific basis for its further development and utilization.关键词
栀子/HPLC/LC-Orbitrap-MS/化学成分/质量控制Key words
Gardeniae Fructus/HPLC/LC-Orbitrap-MS/chemical constituents/quality control分类
医药卫生引用本文复制引用
袁汉文,李云耀,梁玲,吕梦颖,王炜..基于HPLC和LC-Orbitrap-MS的栀子化学成分定性与定量研究[J].湖南中医药大学学报,2023,43(12):2199-2205,7.基金项目
国家重点研发计划"中医药现代化研究"专项"中药国际标准示范研究"(2018YFC1707903) （2018YFC1707903）
湖南省自然科学基金青年项目(2022JJ40318). （2022JJ40318）
