湖南中医药大学学报2023，Vol.43Issue(12)：2256-2263,8.DOI:10.3969/j.issn.1674-070X.2023.12.016
电针调控小胶质细胞极化抑制脑卒中肢体痉挛大鼠脑皮质炎症反应的研究
Electroacupuncture regulates microglia polarization to inhibit inflammatory response in cerebral cortex of rats with post-stroke spasticity
摘要
Abstract
Objective To observe the effects of electroacupuncture at"Yanglingquan"(GB34)and"Quchi"(LI11)on microglia polarization and inflammatory factors in the cerebral cortex of rats with post-stroke spasticity(PSS),so as to explore the possible mechanism of electroacupuncture in alleviating PSS.Methods A PSS model was prepared by the modified middle cerebral artery occlusion(MCAO)suture method + internal capsule injection of N-Methyl-D-aspartic acid(NMDA)receptor.Ten rats were randomly selected as the sham-operated group.After successful modeling,20 rats were selected and randomly subdivided into model group and electroacupuncture group,with 10 rats in each group.The electroacupuncture group was intervened with electroac-upuncture at"Yanglingquan"(GB34)and"Quchi"(LI11)on the affected side,once a day,30 min each time,for 7 consecutive days,while the sham-operated group and the model group were only fixed under the same conditions without any other intervention.The therapeutic effects of electroacupuncture on PSS rats were evaluated through neurological deficit score and electrophysiological tracing;immunofluorescence staining was used to observe the positive expressions of microglia marker ionized calcium binding adapter molecule-1(Iba-1),M1 phenotype marker cluster of differentiation 16(CD16),and M2 phenotype marker cluster of differenti-ation 206(CD206)in ischemic cerebral cortex of rats;ELISA and qPCR were used to determine the content and mRNA expressions of interleukin-6(IL-6),interleukin-1β(IL-1β),and transforming growth factor-β(TGF-β)in the ischemic cerebral cortex of rats.Results Compared with the sham-operated group,the neurological deficit score of the model group significantly increased(P<0.01),the indirect muscle tone markedly decreased(P<0.01),the expressions of Iba-1 and CD16 positive fluorescence intensity significantly elevated(P<0.01),the expression of CD206 positive fluorescence intensity significantly declined(P<0.01),the content and mRNA expressions of IL-6 and IL-1βwere significantly higher(P<0.01),and the content and mRNA expression of TGF-βwere obviously lower(P<0.01).After treatment,compared with the model group,the neurological deficit score of electroacupuncture group decreased(P<0.05),the indirect muscle tone significantly increased(P<0.01),Iba-1 positive fluorescence intensity expression declined(P<0.05),CD16 positive fluorescence intensity expression was obviously lower(P<0.01),CD206 positive fluorescence intensity expression markedly elevated(P<0.01),the content and mRNA expressions of IL-6 and IL-1β decreased(P<0.05),TGF-β content and mRNA expression increased obviously(P<0.01).Conclusion Electroacupuncture at"Yanglingquan"(GB34)and"Quchi"(LI11)can effectively reduce neurological impairment,decrease muscle tone,and alleviate spastic symptoms in PSS rats.The mechanism may be related to inhibiting the activation of microglia,regulating microglia polarization from M1 to M2,and alleviating neuroinflammation.关键词
脑卒中/肢体痉挛/电针/阳陵泉/曲池/小胶质细胞/炎症反应Key words
stroke/limb spasticity/electroacupuncture/"Yanglingquan"(GB34)/"Quchi"(LI11)/microglia/inflammatory response分类
医药卫生引用本文复制引用
黄慧源,易丽贞,黄麟荇,陈瑞雪,绽晟,岳增辉..电针调控小胶质细胞极化抑制脑卒中肢体痉挛大鼠脑皮质炎症反应的研究[J].湖南中医药大学学报,2023,43(12):2256-2263,8.基金项目
国家自然科学基金面上项目(81673886) （81673886）
湖南省自然科学基金面上项目(2023JJ30462) （2023JJ30462）
湖南省研究生科研创新项目(CX20220797) （CX20220797）
湖南中医药大学研究生科研创新项目(2022CX19,2022CX110,2023CX78). （2022CX19,2022CX110,2023CX78）
